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首页> 外文期刊>Infection and immunity >Effective Induction of Acquired Resistance to Listeria monocytogenes by Immunizing Mice with In Vivo-Infected Dendritic Cells
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Effective Induction of Acquired Resistance to Listeria monocytogenes by Immunizing Mice with In Vivo-Infected Dendritic Cells

机译：通过体内感染的树突状细胞免疫小鼠，有效诱导获得性抗单核细胞增生性李斯特菌

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Splenic dendritic cells (DCs) obtained from mice at 48 h after Listeria monocytogenes infection exhibited up-regulation of CD80 and produced higher titers of gamma interferon (IFN-γ) and interleukin-12 (IL-12) than did DCs obtained from uninfected mice. Mice immunized with DCs obtained from mice that had been infected with L. monocytogenes 48 h before acquired host resistance to lethal infection with L. monocytogenes at 4 and 8 weeks. Immunization with DCs from heat-killed L. monocytogenes failed to induce resistance. Acquired antilisterial resistance is specific, since the immunized mice could not be protected from Salmonella enterica serovar Typhimurium infection. Infected DCs stimulated proliferation of naive CD4+ and CD8+ cells in vitro, suggesting that in vivo-infected DCs activate CD8+ T cells, which are critical in acquired antilisterial resistance, as well as CD4+ T cells. When wild-type mice were immunized with DCs from IFN-γ-deficient mice, they were protected against a lethal L. monocytogenes challenge. In contrast, when mice were immunized with DCs from anti-IL-12 p40 monoclonal antibody-injected mice, they failed to gain acquired antilisterial resistance. These results suggest that DC-derived IL-12, but not IFN-γ, may play a critical role in induction of acquired antilisterial resistance. Our present results suggest that splenic DCs obtained from mice infected with L. monocytogenes in vivo may be an effective immunogen with which to induce antigen-specific immunity.
机译：单核细胞增生李斯特氏菌感染后48小时，小鼠脾脏树突状细胞（DC）表现出CD80上调，并产生更高滴度的γ-干扰素（IFN-γ）和白介素12（IL-12）。比从未感染小鼠获得的DC要多。用从被 L感染的小鼠获得的DC免疫的小鼠。单核细胞增生在获得宿主抗 L致死性感染的48小时前。在第4周和第8周出现单核细胞增生病。用热灭活的 L DC进行免疫。单核细胞增生因子未能诱导抗药性。获得性抗李斯特菌耐药性是特异性的，因为不能保护免疫小鼠免受肠炎沙门氏菌血清鼠伤寒感染。感染的DC在体外刺激幼稚CD4 + 和CD8 + 细胞的增殖，这表明体内感染的DC激活了CD8 + T细胞。在获得性抗李斯特菌耐药以及CD4 + T细胞中至关重要。当用来自IFN-γ缺陷型小鼠的DC对野生型小鼠进行免疫接种时，可以保护它们免受致死性 L的侵害。单核细胞增生挑战。相反，当用注射了抗IL-12 p40单克隆抗体的小鼠的DC免疫小鼠时，它们无法获得获得的抗李斯特菌抗性。这些结果表明，DC衍生的IL-12而非IFN-γ可能在诱导获得性抗李斯特菌耐药中起关键作用。我们目前的结果表明，从感染了 L的小鼠获得的脾脏DC。体内的单核细胞增生性单核细胞可能是诱导抗原特异性免疫的有效免疫原。

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