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首页> 外文期刊>Infection and immunity >Lipopolysaccharide-Mimetic Activities of a Toll-Like Receptor 2-Stimulatory Substance(s) in Enterobacterial Lipopolysaccharide Preparations
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Lipopolysaccharide-Mimetic Activities of a Toll-Like Receptor 2-Stimulatory Substance(s) in Enterobacterial Lipopolysaccharide Preparations

机译：肠溶脂多糖制剂中类似Toll受体2-刺激物质的脂多糖模拟活性。

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Lipopolysaccharide (LPS) preparations are known to often contain substances which activate cells through Toll-like receptor 2 (TLR2), and it is suspected that bacterial lipoproteins are responsible for this activation. We compared the mode of action of the TLR2-stimulatory substances with that of a synthetic bacterial lipopeptide (tripalmitoyl-Cys-Ser-Ser-Asn-Ala [Pam3CSSNA]), as well as with that of peptidoglycan. Six out of eight LPS preparations tested induced NF-κB-dependent reporter activity in 293 cells expressing CD14 and TLR2. Phenol extract (PEX) prepared from Escherichia coli LPS by modified phenol extraction induced reporter activity in 293 cells expressing TLR2, and this activity was enhanced by coexpression of CD14, whereas the activity of Pam3CSSNA was not dependent on CD14. The activity of PEX, but not that of Pam3CSSNA or peptidoglycan, was also enhanced by LPS binding protein or serum and blocked by polymyxin B. In addition, the activity of PEX was inhibited by a lipid A precursor (compound 406) in 293 cells expressing CD14 and TLR2. These results indicate that E. coli LPS preparations contain LPS-mimetic TLR2-stimulatory substances which differ from bacterial lipopeptides or peptidoglycan.
机译：已知脂多糖（LPS）制剂通常包含通过Toll样受体2（TLR2）激活细胞的物质，并且怀疑细菌脂蛋白可导致这种激活。我们比较了TLR2刺激物质和合成细菌脂肽（三棕榈酰-Cys-Ser-Ser-Asn-Ala [Pam 3 CSSNA]）的作用方式，以及肽聚糖。在八种LPS制剂中，有六种在表达CD14和TLR2的293细胞中诱导了NF-κB依赖性报道分子活性。通过修饰的苯酚提取法从大肠杆菌 LPS制备的苯酚提取物（PEX）诱导表达TLR2的293细胞中的报告基因活性，并通过共表达CD14增强了该活性，而Pam 3 < / sub> CSSNA不依赖CD14。 LPS结合蛋白或血清可增强PEX的活性，但不增强Pam 3 CSSNA或肽聚糖的活性，而被多粘菌素B阻断。此外，脂质A可抑制PEX的活性。前体（化合物406）在293细胞中表达CD14和TLR2。这些结果表明 E。大肠杆菌LPS制剂中含有模拟LPS的TLR2刺激性物质，不同于细菌脂肽或肽聚糖。

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