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首页> 外文期刊>Infection and immunity >Macrophage-Mediated Germination of Bacillus anthracis Endospores Requires the gerH Operon
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Macrophage-Mediated Germination of Bacillus anthracis Endospores Requires the gerH Operon

机译:巨噬细胞介导的炭疽杆菌内生孢子萌发需要gerH操纵子

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The gerHabc operon of Bacillus anthracis, encoding a gerA-like family member of germinant sensors, was shown to be required for endospore germination in the presence of macrophages and in macrophage-conditioned media. The loss of the germination phenotype in macrophage cultures of B. anthracis gerH-null endospores was restored by complementation in trans with a wild-type copy of gerH expressed under the control of its own promoter. Using endospores from both the parental strain B. anthracis Sterne and an isogenic gerH-null strain, we partially characterized germinants secreted by macrophages into the extracellular medium.
机译:炭疽芽孢杆菌的 gerHabc 操纵子,编码发芽传感器的 gerA 样家族成员,被证明是存在内孢子萌发所必需的巨噬细胞和巨噬细胞条件培养基中。在 B巨噬细胞培养中发芽表型的丧失。炭疽菌gerH -无效的内生孢子通过在 trans 中与野生型拷贝的 gerH 在其自身启动子的控制下互补而恢复。使用来自两个亲本菌株B的内生孢子。炭疽Sterne和同基因的 gerH -null菌株,我们将巨噬细胞分泌的发芽细胞部分表征为细胞外培养基。

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