Human β-Defensin 2 Is Expressed and Associated with Mycobacterium tuberculosis during Infection of Human Alveolar Epithelial Cells

Bruno Rivas-Santiago; Stephan K. Schwander; Carmen Sarabia; Gill Diamond; Marcia E. Klein-Patel; Rogelio Hernandez-Pando; Jerrold J. Ellner; Eduardo Sada

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Human β-Defensin 2 Is Expressed and Associated with Mycobacterium tuberculosis during Infection of Human Alveolar Epithelial Cells

机译：在人肺泡上皮细胞感染过程中，人β-防御素2表达并与结核分枝杆菌相关。

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To determine the role of human β-defensin 2 (HBD-2) in human tuberculosis, we studied the in vitro induction of HBD-2 gene expression by Mycobacterium tuberculosis H37Rv infection in the human lung epithelial cell line A549, in alveolar macrophages (AM), and in blood monocytes (MN) by reverse transcription-PCR. We also studied the induction of HBD-2 gene expression by mannose lipoarabinomannan (manLAM) from M. tuberculosis. Intracellular production of HBD-2 peptide was detected by immunocytochemistry and electron microscopy. Our results demonstrated that there was induction of HBD-2 mRNA in A549 cells after infection with M. tuberculosis at various multiplicities of infection (MOI) and that there was stimulation with manLAM. AM expressed the HBD-2 gene only at a high MOI with M. tuberculosis. MN did not express HBD-2 at any of the experimental M. tuberculosis MOI. Immunostaining revealed the presence of intracellular HBD-2 peptide in A549 cells following infection with M. tuberculosis, and the staining was more intense in areas where there were M. tuberculosis clusters. By using electron microscopy we also demonstrated production of HBD-2 after M. tuberculosis infection and adherence of HBD-2 to the membranes of M. tuberculosis. Alveolar epithelial cells are among the first cells to encounter M. tuberculosis following aerogenic infection. As HBD-2 has been shown to control growth of M. tuberculosis and has chemotactic activity, our results suggest that HBD-2 induction by M. tuberculosis may have a role in the pathogenesis of human tuberculosis.

机译：为了确定人β-防御素2（HBD-2）在人结核中的作用，我们研究了结核分枝杆菌 H37Rv感染在人肺上皮细胞系中体外诱导HBD-2基因表达的情况。通过逆转录PCR检测肺泡巨噬细胞（AM）和血液单核细胞（MN）中的A549。我们还研究了 M的甘露糖脂阿拉伯糖甘露聚糖（manLAM）对HBD-2基因表达的诱导作用。结核病。通过免疫细胞化学和电子显微镜检测HBD-2肽的细胞内产生。我们的结果表明，感染 M后，A549细胞中有HBD-2 mRNA的诱导。各种感染（MOI）引起的结核病，并且manLAM有刺激作用。 AM仅以 M的高MOI表达HBD-2基因。结核病。 MN在任何实验性M均未表达HBD-2。结核病。免疫染色显示，感染 M后，A549细胞中存在细胞内HBD-2肽。结核，并且在有 M的区域，染色更强烈。结核簇。通过使用电子显微镜，我们还证明了 M后HBD-2的产生。结核感染和HBD-2粘附在 M膜上。结核病。肺泡上皮细胞是最早遇到M的细胞。感染后发生肺结核。因为已经显示出HBD-2控制 M的生长。结核并具有趋化活性，我们的结果提示 M诱导HBD-2。结核病可能在人类结核病的发病机理中起作用。 展开▼

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《Infection and immunity》 |2005年第8期|共7页

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Bruno Rivas-Santiago; Stephan K. Schwander; Carmen Sarabia; Gill Diamond; Marcia E. Klein-Patel; Rogelio Hernandez-Pando; Jerrold J. Ellner; Eduardo Sada;
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中图分类医学免疫学;

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1. Human β-Defensin 2 Is Expressed and Associated with Mycobacterium tuberculosis during Infection of Human Alveolar Epithelial Cells [J] . Bruno Rivas-Santiago, Stephan K. Schwander, Carmen Sarabia, Infection and immunity . 2005,第8期

机译：在人肺泡上皮细胞感染过程中，人β-防御素2表达并与结核分枝杆菌相关。

2. Expression of Cathelicidin LL-37 during Mycobacterium tuberculosis Infection in Human Alveolar Macrophages, Monocytes, Neutrophils, and Epithelial Cells [J] . Bruno Rivas-Santiago, Rogelio Hernandez-Pando, Claudia Carranza, Infection and immunity . 2008,第3期

机译：人肺泡巨噬细胞，单核细胞，中性粒细胞和上皮细胞在结核分枝杆菌感染过程中Cathelicidin LL-37的表达

3. Mycobacterium tuberculosis infection causes different levels of apoptosis and necrosis in human macrophages and alveolar epithelial cells [J] . Lia Danelishvili, Jeffery McGarvey, Yong-jun Li, Cellular microbiology . 2003,第9期

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4. Differences in form stability between human non-tumorous alveolar epithelial cells type 2 and alveolar carcinoma cells under biaxial stretching [C] . S.J. Schliefimann, K. Hohne, A. Charra European Conferenc of the International Federation for Medical and Biological Engineering . 2008

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5. Chemokine production by human alveolar epithelial cells in response to Mycobacterium tuberculosis infection. [D] . Sequeira, Patricia Carvalho. 2007

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6. Human β-Defensin 2 Is Expressed and Associated with Mycobacterium tuberculosis during Infection of Human Alveolar Epithelial Cells [O] . Bruno Rivas-Santiago, Stephan K. Schwander, Carmen Sarabia, 2005

机译：在人肺泡上皮细胞感染过程中人β-防御素2表达并与结核分枝杆菌相关。

7. Human β-Defensin 2 Is Expressed and Associated with Mycobacterium tuberculosis during Infection of Human Alveolar Epithelial Cells [O] . Rivas-Santiago, Bruno, Schwander, Stephan K., Sarabia, Carmen, 2005

机译：在人肺泡上皮细胞感染过程中，人β-防御素2表达并与结核分枝杆菌相关。

8. Cryptosporidium Infection of Human Intestinal Epithelial Cells Increases Expression of Osteoprotegerin: A Novel Mechanism for Evasion of Host Defenses. [R] . Castellanos-Gonzalez, A., Yancey, L. S., Wang, H., 2008

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Human β-Defensin 2 Is Expressed and Associated with Mycobacterium tuberculosis during Infection of Human Alveolar Epithelial Cells

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