• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Differences in Chlamydia trachomatis Serovar E Growth Rate in Polarized Endometrial and Endocervical Epithelial Cells Grown in Three-Dimensional Culture
【24h】

Differences in Chlamydia trachomatis Serovar E Growth Rate in Polarized Endometrial and Endocervical Epithelial Cells Grown in Three-Dimensional Culture

机译：三维培养中极化的子宫内膜和宫颈上皮细胞中沙眼衣原体血清型E增长率的差异

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In vitro studies of obligate intracellular chlamydia biology and pathogenesis are highly dependent on the use of experimental models and growth conditions that mimic the mucosal architecture and environment these pathogens encounter during natural infections. In this study, the growth of Chlamydia trachomatis genital serovar E was monitored in mouse fibroblast McCoy cells and compared to more relevant host human epithelial endometrium-derived HEC-1B and cervix-derived HeLa cells, seeded and polarized on collagen-coated microcarrier beads, using a three-dimensional culture system. Microscopy analysis of these cell lines prior to infection revealed morphological differences reminiscent of their in vivo architecture. Upon infection, early chlamydial inclusion distribution was uniform in McCoy cells but patchy in both epithelial cell lines. Although no difference in chlamydial attachment to or entry into the two genital epithelial cell lines was noted, active bacterial genome replication and transcription, as well as initial transformation of elementary bodies to reticulate bodies, were detected earlier in HEC-1B than in HeLa cells, suggesting a faster growth, which led to higher progeny counts and titers in HEC-1B cells upon completion of the developmental cycle. Chlamydial development in the less relevant McCoy cells was very similar to that in HeLa cells, although higher progeny counts were obtained. In conclusion, this three-dimensional bead culture system represents an improved model for harvesting large quantities of infectious chlamydia progeny from their more natural polarized epithelial host cells.
机译：专性细胞内衣原体生物学和发病机制的体外研究高度依赖于模拟这些病原体在自然感染过程中遇到的粘膜结构和环境的实验模型和生长条件的使用。在这项研究中，在小鼠成纤维细胞McCoy细胞中监测了沙眼衣原体生殖器血清型E的生长，并将其与更相关的宿主人上皮子宫内膜来源的HEC-1B和子宫颈来源的HeLa细胞进行了比较使用三维培养系统在胶原蛋白包被的微载体珠子上感染前对这些细胞系的显微镜分析表明其形态差异让人联想到它们的体内结构。感染后，早期的衣原体包涵体分布在McCoy细胞中是均匀的，但在两个上皮细胞系中都斑片状。尽管未发现衣原体附着或进入两种生殖器上皮细胞系的差异，但在HEC-1B中检测到的活性细菌基因组复制和转录以及基本体向网状体的初始转化要早于HeLa细胞，提示更快的生长，导致发育周期完成后，HEC-1B细胞的后代计数和滴度更高。尽管获得了更高的后代计数，但相关性较低的McCoy细胞中的衣原体发育与HeLa细胞中的衣原体发育非常相似。总之，此三维珠培养系统代表了一种改进的模型，可从其更自然的极化上皮宿主细胞中收获大量感染性衣原体子代。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号