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首页> 外文期刊>Infection and immunity >The Sca2 Autotransporter Protein from Rickettsia conorii Is Sufficient To Mediate Adherence to and Invasion of Cultured Mammalian Cells
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The Sca2 Autotransporter Protein from Rickettsia conorii Is Sufficient To Mediate Adherence to and Invasion of Cultured Mammalian Cells

机译：来自康氏立克次体的Sca2自转运蛋白足以介导对培养的哺乳动物细胞的粘附和侵袭

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Obligate intracellular bacteria of the genus Rickettsia must adhere to and invade the host endothelium in order to establish an infection. These processes require the interaction of rickettsial surface proteins with mammalian host cell receptors. A previous bioinformatic analysis of sequenced rickettsial species identified a family of at least 17 predicted “surface cell antigen” (sca) genes whose products resemble autotransporter proteins. Two members of this family, rOmpA and rOmpB of spotted fever group (SFG) rickettsiae have been identified as adhesion and invasion factors, respectively; however, little is known about the putative functions of the other sca gene products. An intact sca2 gene is found in the majority of pathogenic SFG rickettsiae and, due to its sequence conservation among these species, we predict that Sca2 may play an important function at the rickettsial surface. Here we have shown that sca2 is transcribed and expressed in Rickettsia conorii and have used a heterologous gain-of-function assay in E. coli to determine the putative role of Sca2. Using this system, we have demonstrated that expression of Sca2 at the outer membrane of nonadherent, noninvasive E. coli is sufficient to mediate adherence to and invasion of a panel of mammalian cells, including endothelial cells. Furthermore, soluble Sca2 protein is capable of diminishing R. conorii invasion of cultured mammalian cells. This is the first evidence that Sca2 participates in the interaction between SFG rickettsiae and host cells and suggests that in addition to other surface proteins, Sca2 may play a critical role in rickettsial pathogenesis.
机译：立克次氏体属的专性细胞内细菌必须附着并侵入宿主内皮才能建立感染。这些过程需要立克次体表面蛋白与哺乳动物宿主细胞受体的相互作用。先前对测序的立克次氏菌物种进行的生物信息学分析确定了至少17个预测的“表面细胞抗原”（ sca ）基因家族，其产物类似于自转运蛋白。该家族的两个成员，点状发热立克次体（SFG）立克次体的rOmpA和rOmpB被分别确定为黏附因子和入侵因子。但是，对其他 sca 基因产物的推定功能知之甚少。在大多数致病性SFG立克次体中发现了完整的 sca2 基因，由于其在这些物种中的序列保守性，我们预测Sca2可能在立克次体表面起重要作用。在这里，我们显示了 sca2 在 Rickettsia conorii 中转录并表达，并在 E中使用了异源功能获得分析。大肠杆菌来确定Sca2的假定作用。使用该系统，我们已经证明了Sca2在非粘附性，非侵入性 E外膜上的表达。大肠杆菌足以介导一系列哺乳动物细胞（包括内皮细胞）的粘附和侵袭。此外，可溶性Sca2蛋白能够降低 R。 conorii 对培养的哺乳动物细胞的侵袭。这是Sca2参与SFG立克次体与宿主细胞之间相互作用的第一个证据，表明除其他表面蛋白外，Sca2可能在立克次氏体发病中起关键作用。

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