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首页> 外文期刊>Infection and immunity >Contribution of Autolysin and Sortase A during Enterococcus faecalis DNA-Dependent Biofilm Development
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Contribution of Autolysin and Sortase A during Enterococcus faecalis DNA-Dependent Biofilm Development

机译：粪肠球菌DNA依赖性生物膜发育过程中自溶素和分选酶A的贡献。

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摘要

Biofilm production is a major attribute of Enterococcus faecalis clinical isolates. Although some factors, such as sortases, autolysin, and extracellular DNA (eDNA), have been associated with E. faecalis biofilm production, the mechanisms underlying the contributions of these factors to this process have not been completely elucidated yet. In this study we define important roles for the major E. faecalis autolysin (Atn), eDNA, and sortase A (SrtA) during the developmental stages of biofilm formation under static and hydrodynamic conditions. Deletion of srtA affects the attachment stage and results in a deficiency in biofilm production. Atn-deficient mutants are delayed in biofilm development due to defects in primary adherence and DNA release, which we show to be particularly important during the accumulative phase for maturation and architectural stability of biofilms. Confocal laser scanning and freeze-dry electron microscopy of biofilms grown under hydrodynamic conditions revealed that E. faecalis produces a DNase I-sensitive fibrous network, which is important for biofilm stability and is absent in atn-deficient mutant biofilms. This study establishes the stage-specific requirements for SrtA and Atn and demonstrates a role for Atn in the pathway leading to DNA release during biofilm development in E. faecalis.
机译：生物膜生产是粪肠球菌临床分离株的主要属性。尽管某些因素（例如分选酶，自溶素和细胞外DNA（eDNA））与 E相关。粪便生物膜的产生，这些因素对这一过程的贡献所起的作用机理尚未完全阐明。在这项研究中，我们定义了主要 E的重要角色。静态和水动力条件下生物膜形成过程中的粪便自溶素（Atn），eDNA和分选酶A（SrtA）。 srtA 的缺失会影响附着阶段，并导致生物膜生产不足。 Atn缺陷突变体由于主要粘附和DNA释放缺陷而延迟了生物膜的发育，这表明在累积阶段对于生物膜的成熟和建筑稳定性而言，这一点尤其重要。在水动力条件下生长的生物膜的共聚焦激光扫描和冻干电子显微镜揭示了 E。粪便会产生DNase I敏感的纤维网，这对生物膜的稳定性很重要，而在 atn 缺失的突变生物膜中却不存在。这项研究确定了SrtA和Atn的特定阶段需求，并证明了Atn在导致Eem生物膜发育过程中DNA释放的途径中的作用。粪便。

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