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首页> 外文期刊>Infection and immunity >SarZ Promotes the Expression of Virulence Factors and Represses Biofilm Formation by Modulating SarA and agr in Staphylococcus aureus
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SarZ Promotes the Expression of Virulence Factors and Represses Biofilm Formation by Modulating SarA and agr in Staphylococcus aureus

机译：SarZ通过调节金黄色葡萄球菌中的SarA和agr促进毒力因子的表达并抑制生物膜的形成

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摘要

Staphylococcus aureus is a remarkably adaptable organism capable of multiple modes of growth in the human host, as a part of the normal flora, as a pathogen, or as a biofilm. Many of the regulatory pathways governing these modes of growth are centered on the activities of two regulatory molecules, the DNA binding protein SarA and the regulatory RNAIII effector molecule of the agr system. Here, we describe the modulation of these regulators and their downstream target genes by SarZ, a member of the SarA/MarR family of transcriptional regulators. Transcriptional and phenotypic analyses of a sarZ mutant demonstrated that the decreased transcription of mgrA and the agr RNAIII molecule was accompanied by increased transcription of spa (protein A) and downregulation of hla (alpha-hemolysin) and sspA (V8 protease) transcripts when compared to its isogenic parent. The decrease in protease activity was also associated with an increase in SarA expression. Consistent with an increase in SarA levels, the sarZ mutant displayed an enhanced ability to form biofilms. Together, our results indicate that SarZ may be an important regulator governing the dissemination phase of S. aureus infections, as it promotes toxin expression while repressing factors required for biofilm formation.
机译：金黄色葡萄球菌是一种非常适应的生物，能够在人类宿主中以多种生长模式生长，作为正常菌群的一部分，作为病原体或作为生物膜。控制这些生长方式的许多调节途径都集中在两个调节分子的活性上，这两个分子是DNA结合蛋白SarA和 agr 系统的调节RNAIII效应分子。在这里，我们描述了转录调控因子SarA / MarR家族的成员SarZ对这些调控因子及其下游靶基因的调控。对 sarZ 突变体的转录和表型分析表明， mgrA 和 agr RNAIII分子的转录减少伴随着的转录增加spa （蛋白A）和 hla （α-溶血素）和 sspA （V8蛋白酶）转录本的同基因亲本相比。蛋白酶活性的降低也与SarA表达的增加有关。与SarA水平升高一致， sarZ 突变体显示出增强的生物膜形成能力。总之，我们的结果表明，SarZ可能是控制 S传播阶段的重要调节剂。金黄色葡萄球菌感染，因为它促进毒素表达，同时抑制生物膜形成所需的因子。

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