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首页> 外文期刊>Infection and immunity >Examination of Campylobacter jejuni Putative Adhesins Leads to the Identification of a New Protein, Designated FlpA, Required for Chicken Colonization
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Examination of Campylobacter jejuni Putative Adhesins Leads to the Identification of a New Protein, Designated FlpA, Required for Chicken Colonization

机译：空肠弯曲杆菌推定粘附素的检测导致鉴定新的蛋白质，称为FlpA，是鸡定殖所必需的

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Campylobacter jejuni colonization of chickens is presumably dependent upon multiple surface-exposed proteins termed adhesins. Putative C. jejuni adhesins include CadF, CapA, JlpA, major outer membrane protein, PEB1, Cj1279c, and Cj1349c. We examined the genetic relatedness of 97 C. jejuni isolates recovered from human, poultry, bovine, porcine, ovine, and canine sources by multilocus sequence typing (MLST) and examined their profile of putative adhesin-encoding genes by dot blot hybridization. To assess the individual contribution of each protein in bacterium-host cell adherence, the C. jejuni genes encoding the putative adhesins were disrupted by insertional mutagenesis. The phenotype of each mutant was judged by performing in vitro cell adherence assays with chicken LMH hepatocellular carcinoma epithelial cells and in vivo colonization assays with broiler chicks. MLST analysis indicated that the C. jejuni isolates utilized in this study were genetically diverse. Dot blot hybridization revealed that the C. jejuni genes encoding the putative adhesins, with the exception of capA, were conserved among the isolates. The C. jejuni CadF, CapA, Cj1279c, and Cj1349c proteins were found to play a significant role in the bacterium's in vitro adherence to chicken epithelial cells, while CadF, PEB1, and Cj1279c were determined to play a significant role in the bacterium's in vivo colonization of broiler chicks. Collectively, the data indicate that Cj1279c is a novel adhesin. Because Cj1279c harbors fibronectin type III domains, we designated the protein FlpA, for fibronectin-like protein A.
机译：空肠弯曲杆菌的定殖可能依赖于多种表面粘附蛋白，即粘附素。推定的C。空肠粘附素包括CadF，CapA，JlpA，主要外膜蛋白，PEB1，Cj1279c和Cj1349c。我们检查了97 C的遗传相关性。通过多基因座序列分型（MLST）从人，家禽，牛，猪，绵羊和犬源中回收的空肠分离株，并通过斑点印迹杂交检查了假定的粘附素编码基因的分布。为了评估每种蛋白质对细菌-宿主细胞粘附的贡献， C。插入诱变破坏了编码假定的粘附素的空肠基因。通过用鸡LMH肝细胞癌上皮细胞进行体外细胞粘附测定和用肉鸡进行体内定植测定来判断每种突变体的表型。 MLST分析表明 C。本研究中使用的空肠分离物在遗传上是多样的。点印迹杂交揭示了 C。除 capA 外，编码推定的粘附素的空肠基因被保守。 C。空肠中的CadF，CapA，Cj1279c和Cj1349c蛋白在细菌对鸡上皮细胞的体外粘附中起重要作用，而CadF，PEB1和Cj1279c被确定在细菌的鸡上皮细胞中起重要作用。肉鸡体内定殖。总体而言，数据表明Cj1279c是新型粘附素。由于Cj1279c具有纤连蛋白III型结构域，因此我们将蛋白FlpA命名为 f ibronectin- l ike p rotein A 。

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