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首页> 外文期刊>Infection and immunity >Intraspecies and Temperature-Dependent Variations in Susceptibility of Yersinia pestis to the Bactericidal Action of Serum and to Polymyxin B
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Intraspecies and Temperature-Dependent Variations in Susceptibility of Yersinia pestis to the Bactericidal Action of Serum and to Polymyxin B

机译：鼠疫耶尔森氏菌对血清杀菌活性和多粘菌素B的种内和温度依赖性变化

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Lipopolysaccharide (LPS) structure impacts the bactericidal action of cationic peptides, such as polymyxin B (PMB), and sensitivity to killing by normal human serum (NHS). Cultivation of different subspecies strains of Yersinia pestis isolated from unrelated geographic origins at various temperatures (mammals, 37°C; fleas, 25°C; or winter hibernation, 6°C) affects LPS composition and structure. We tested the susceptibilities of various strains of Y. pestis grown at these different temperatures to PMB and serum bactericidal killing. Both properties varied significantly in response to temperature changes. In Y. pestis subsp. pestis (the main subspecies causing human plague), high levels of resistance to PMB and NHS were detected at 25°C. However, at the same temperature, Y. pestis subsp. caucasica was highly sensitive to PMB. At both of the extreme temperatures, all strains were highly susceptible to PMB. At 25°C and 37°C, Y. pestis subsp. caucasica strain 1146 was highly susceptible to the bactericidal activity of 80% NHS. All Y. pestis strains studied were able to grow in heat-inactivated human serum or in 80% normal mouse serum. At 6°C, all strains were highly sensitive to NHS. Variations in the PMB resistance of different bacterial cultures related to both the content of cationic components (4-amino-4-deoxyarabinose in lipid A and glycine in the core) and a proper combination of terminal monosaccharides in the LPS. The NHS resistance correlated with an elevated content of N-acetylglucosamine in the LPS. Structural variation in the LPS of Y. pestis correlates with the organism's ability to resist innate immunity in both fleas and mammals.
机译：脂多糖（LPS）的结构影响阳离子肽（如多粘菌素B（PMB））的杀菌作用，以及对正常人血清（NHS）杀灭的敏感性。从不同地理起源分离的鼠疫耶尔森菌不同亚种菌株在不同温度（哺乳动物，37°C;跳蚤，25°C;或冬眠，6°C）下的培养会影响脂多糖的组成和结构。我们测试了 Y的各种菌株的敏感性。在不同温度下生长的鼠疫杆菌对PMB和血清具有杀菌作用。两种性质均响应温度变化而显着变化。在 Y。瘟疫子空间 pestis （引起人类鼠疫的主要亚种）在25°C时检测到对PMB和NHS的高水平抗药性。但是，在相同温度下， Y。瘟疫子空间 caucasica 对PMB高度敏感。在两个极端温度下，所有菌株均高度易受PMB影响。在25°C和37°C下， Y。瘟疫子空间 caucasica 菌株1146对80％NHS的杀菌活性高度敏感。全部 Y。所研究的瘟疫菌株能够在热灭活的人血清或80％正常小鼠血清中生长。在6°C下，所有菌株均对NHS高度敏感。不同细菌培养物对PMB的抗性变化与阳离子成分（脂质A中的4-氨基-4-脱氧阿拉伯糖和核心中的甘氨酸）的含量以及LPS中末端单糖的适当组合有关。 NHS抗性与LPS中 N -乙酰氨基葡糖含量的升高有关。 Y的LPS的结构变异。鼠疫与该生物抵抗跳蚤和哺乳动物固有免疫力的能力有关。

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