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首页> 外文期刊>Infection and immunity >Protection against Leptospira interrogans Sensu Lato Challenge by DNA Immunization with the Gene Encoding Hemolysin-Associated Protein 1
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Protection against Leptospira interrogans Sensu Lato Challenge by DNA Immunization with the Gene Encoding Hemolysin-Associated Protein 1

机译：通过编码与溶血素相关蛋白1基因编码的DNA免疫来对抗钩端螺旋体Sensu Lato挑战。

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The use of DNA constructs encoding leptospiral proteins is a promising new approach for vaccination against leptospirosis. In previous work we determined that immunization with hemolysis-associated protein 1 (Hap1) (LipL32) expressed by adenovirus induced significant protection against a virulent Leptospira challenge in gerbils. To avoid the use of the adenovirus vector, we checked for clinical protection against lethal challenge by DNA vaccination. A DNA vaccine expressing Hap1 was designed to enhance the direct gene transfer of this protein into gerbils. A challenge was performed 3 weeks after the last immunization with a virulent strain of serovar canicola. Our results show that the cross-protective effect with pathogenic strains of Leptospira, shared by Hap1, could be mediated by the DNA plasmid vector. This finding should facilitate the design and development of a new generation of vaccines against bacteria, particularly Leptospira interrogans sensu lato.
机译：编码钩端螺旋体蛋白的DNA构建体的使用是针对钩端螺旋体病接种的有希望的新方法。在先前的工作中，我们确定了用腺病毒表达的溶血相关蛋白1（Hap1）（LipL32）进行的免疫诱导了对沙土鼠强力 Leptospira 攻击的显着保护作用。为避免使用腺病毒载体，我们检查了针对DNA疫苗接种对致命性攻击的临床保护。表达Hap1的DNA疫苗被设计来增强该蛋白向沙土鼠的直接基因转移。在最后一次免疫后3周，用强毒血清卡尼科拉菌株进行攻击。我们的研究结果表明，DNA质粒载体可以介导Hap1与 Leptospira 致病菌株的交叉保护作用。这一发现应有助于设计和开发新一代针对细菌的疫苗，尤其是问号螺旋藻 sensu lato。

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