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首页> 外文期刊>Infection and immunity >Efficacy of Vaccination of Calves against Hemorrhagic Septicemia with a Live aroA Derivative of Pasteurella multocida B:2 by Two Different Routes of Administration
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Efficacy of Vaccination of Calves against Hemorrhagic Septicemia with a Live aroA Derivative of Pasteurella multocida B:2 by Two Different Routes of Administration

机译：通过两种不同的给药途径对多杀性巴斯德氏菌B：2的活aroA衍生物进行犊牛预防出血性败血病的效果

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Two groups of four calves each were immunized either intramuscularly (i.m. vaccinated) or intranasally (i.n. vaccinated) at 2 and 6 weeks of age with ca. 109 CFU of a derivative of P. multocida serotype B:2 strain 85020 containing a deletion in the aroA gene (strain JRMT12). Both groups of calves and three unvaccinated control calves were challenged subcutaneously at 8 weeks of age with ca. 107 CFU of the wild-type 85020 strain. The first and second vaccinations caused a significant pyrexia and increase in the mean demeanor score (P < 0.05) in i.m. but not i.n. vaccinated calves. Serum agglutinating activity against whole cells of P. multocida strain 85020 and immunoglobulin G antibody concentrations increased after the second vaccination in i.m. but not in i.n. vaccinated animals, and this difference was statistically significant (P < 0.05). Concentrations of serum amyloid A (SAA) increased significantly 3 h after both the primary (P < 0.05) and booster (P < 0.001) i.m. vaccinations, but not in i.n. vaccinated calves. All four i.m. vaccinated calves were solidly immune to challenge with wild-type P. multocida B:2. However, the mean rectal temperatures, demeanor scores, and serum SAA concentrations of i.n. vaccinated and control calves increased significantly (P < 0.01). Three i.n. vaccinated and two control calves were killed for humane reasons within 14 h postchallenge, and postmortem examination revealed pathological lesions consistent with hemorrhagic septicemia. These data showed that the aroA mutant strain, given i.m. as two doses 4 weeks apart, acted as an effective live-attenuated vaccine strain to protect calves against challenge with the virulent parent strain.
机译：在第2周和第6周龄时，分别对两组的四只小牛进行肌肉内（免疫接种）或鼻内（免疫接种）免疫。 P衍生物的10 9 CFU。含有 aroA 基因缺失的多杀性巴氏杆菌B：2菌株85020（JRMT12菌株）。两组小牛和三只未接种疫苗的对照小牛均在8周龄时皮下攻击，约。野生型85020菌株的10 7 CFU。第一次和第二次疫苗接种导致i.m的明显发热和平均举止得分增加（ P <0.05）。但不是接种牛犊。针对 P的全细胞的血清凝集活性。接种第二次疫苗后，多杀霉菌85020株和免疫球蛋白G抗体浓度增加。但不在i.n.疫苗接种的动物，这一差异具有统计学意义（ P <0.05）。初次（ P <0.05）和加强免疫（ P <0.001）后3 h，血清淀粉样蛋白A（SAA）的浓度显着增加。接种疫苗，但不能在i.n.接种牛犊。所有四个上午接种牛犊对野生型 P具有较强的免疫攻击能力。 B.2。但是，平均直肠温度，举止得分和血清SAA浓度为i.n。接种过的小牛和对照牛犊明显增加（ P <0.01）。三世接种疫苗后，在人身攻击后14小时内，出于人道原因杀死了两只对照小牛，死后检查发现病理性病变与出血性败血病相符。这些数据表明，在给定的时间内， aroA 突变株。间隔4周分两次服用，可作为有效的减毒活疫苗株，以保护犊牛免受强毒母株的攻击。

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