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首页> 外文期刊>Infection and immunity >Identification of CsrC and Characterization of Its Role in Epithelial Cell Invasion in Salmonella enterica Serovar Typhimurium
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Identification of CsrC and Characterization of Its Role in Epithelial Cell Invasion in Salmonella enterica Serovar Typhimurium

机译：CsrC的鉴定及其在肠炎沙门氏菌鼠伤寒沙门氏菌上皮细胞侵袭中的作用

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The csr regulatory system of Salmonella regulates the expression of the genes of Salmonella pathogenicity island 1 (SPI1) required for the invasion of epithelial cells. This system consists of the posttranscriptional regulator CsrA and an untranslated regulatory RNA, CsrB, that opposes the action of CsrA. Here we identify and characterize the role of a second regulatory RNA, CsrC, whose ortholog was discovered previously in Escherichia coli. We show that a mutant of csrC has only mild defects in invasion and the expression of SPI1 genes, as does a mutant of csrB, but that a double csrB csrC mutant is markedly deficient in these properties, suggesting that the two regulatory RNAs play redundant roles in the control of invasion. We further show that CsrC, like CsrB, is controlled by the BarA/SirA two-component regulator but that a csrB csrC mutant exhibits a loss of invasion equivalent to that of a barA or sirA mutant, indicating that much of the effect of BarA/SirA on invasion functions through its control of CsrB and CsrC. In addition to their control by BarA/SirA, each regulatory RNA is also controlled by other components of the csr system. The loss of csrB was found to increase the level of CsrC by sevenfold, while the loss of csrC increased CsrB by nearly twofold. Similarly, the overexpression of csrA increased CsrC by nearly 11-fold and CsrB by 3-fold and also significantly increased the stability of both RNAs.
机译：沙门氏菌的csr调控系统调节上皮细胞入侵所需的沙门氏菌致病岛1（SPI1）基因的表达。该系统由转录后调节子CsrA和与CsrA作用相反的未翻译的调节RNA CsrB组成。在这里，我们确定并表征了第二种调节性RNA CsrC的作用，其直系同源物先前是在大肠杆菌中发现的。我们显示， csrC 的突变体与 csrB 的突变体一样，在侵袭和SPI1基因表达方面仅存在轻度缺陷，但是存在一个双重的 csrB csrC 突变体在这些特性上明显不足，表明这两个调控RNA在入侵控制中起着多余的作用。我们进一步表明，CsrC与CsrB一样，受BarA / SirA两组分调节剂的控制，但是 csrB csrC 突变体表现出的侵害损失与 barA 或 sirA 突变体，表明BarA / SirA通过控制CsrB和CsrC对入侵功能发挥了很大作用。除了受BarA / SirA的控制外，每种调节性RNA也受csr系统的其他组件控制。发现 csrB 的丢失使CsrC的水平增加了七倍，而 csrC 的丢失使CsrB的水平增加了近两倍。同样， csrA 的过表达使CsrC增加了近11倍，CsrB增加了3倍，并且还显着提高了两种RNA的稳定性。

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