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首页> 外文期刊>Infection and immunity >Brucella abortus Transits through the Autophagic Pathway and Replicates in the Endoplasmic Reticulum of Nonprofessional Phagocytes
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Brucella abortus Transits through the Autophagic Pathway and Replicates in the Endoplasmic Reticulum of Nonprofessional Phagocytes

机译：流产布鲁氏菌通过自噬途径转运并在非专业吞噬细胞的内质网中复制

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Brucella abortus is an intracellular pathogen that replicates within a membrane-bounded compartment. In this study, we have examined the intracellular pathway of the virulent B. abortus strain 2308 (S2308) and the attenuated strain 19 (S19) in HeLa cells. At 10 min after inoculation, both bacterial strains are transiently detected in phagosomes characterized by the presence of early endosomal markers such as the early endosomal antigen 1. At ～1 h postinoculation, bacteria are located within a compartment positive for the lysosome-associated membrane proteins (LAMPs) and the endoplasmic reticulum (ER) marker sec61β but negative for the mannose 6-phosphate receptors and cathepsin D. Interestingly, this compartment is also positive for the autophagosomal marker monodansylcadaverin, suggesting that S2308 and S19 are located in autophagic vacuoles. At 24 h after inoculation, attenuated S19 is degraded in lysosomes, while virulent S2308 multiplies within a LAMP- and cathepsin D-negative but sec61β- and protein disulfide isomerase-positive compartment. Furthermore, treatment of infected cells with the pore-forming toxin aerolysin from Aeromonas hydrophila causes vacuolation of the bacterial replication compartment. These results are compatible with the hypothesis that pathogenic B. abortus exploits the autophagic machinery of HeLa cells to establish an intracellular niche favorable for its replication within the ER.
机译：流产布鲁氏菌是一种细胞内病原体，可在有膜限制的隔室内复制。在这项研究中，我们检查了有毒的 B的细胞内途径。 HeLa细胞中流产的2308株（S2308）和减毒的19株（S19）。接种后10分钟，在特征为早期内体标记物（例如早期内体抗原1）的吞噬体中瞬时检测到两种细菌菌株。在接种后约1小时，细菌位于溶酶体相关膜蛋白阳性的隔室中。 （LAMPs）和内质网（ER）标记sec61β，但对甘露糖6磷酸受体和组织蛋白酶D阴性。有趣的是，该隔室对自噬体标记单丹磺酰尸胺也呈阳性，表明S2308和S19位于自噬液泡中。接种后24小时，减毒的S19在溶酶体中降解，而强毒的S2308在LAMP和组织蛋白酶D阴性但在sec61β和蛋白质二硫键异构酶阳性区室中繁殖。此外，用嗜水气单胞菌的造孔毒素气溶素对感染的细胞进行处理会导致细菌复制区的空泡化。这些结果与致病性 B假说相符。流产利用HeLa细胞的自噬机制建立了一个有利于其在ER中复制的细胞内生态位。

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