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首页> 外文期刊>Infection and immunity >Role of Hemolysin BL in the Pathogenesis of Extraintestinal Bacillus cereus Infection Assessed in an Endophthalmitis Model
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Role of Hemolysin BL in the Pathogenesis of Extraintestinal Bacillus cereus Infection Assessed in an Endophthalmitis Model

机译：溶血素BL在眼内炎模型中评估肠蜡状芽胞杆菌感染的发病机理中的作用

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Bacillus cereus is a rare cause of serious human infection but, paradoxically, causes one of the most severe posttraumatic or endogenous infections of the eye, endophthalmitis, which frequently results in blindness. The virulence of B. cereus endophthalmitis historically has been attributed to toxin production. We therefore sought to examine the contribution of the dermonecrotic toxin, hemolysin BL, to the pathogenesis of B. cereus infection in an endophthalmitis system that is highly amenable to study. The pathogenesis of infection resulting from intravitreal injection of 102 CFU of either a clinical ocular isolate of B. cereus producing hemolysin BL (HBL+) or an isogenic mutant in this trait (HBL?) was assessed bacteriologically and by slit lamp biomicroscopy, electroretinography, histology, and inflammatory cell enumeration. Both HBL+ and HBL? strains evoked severe intraocular inflammatory responses as early as 12 h postinfection, with complete loss of retinal responsiveness by 12 h. The infections caused by both strains spread of the infection to adjacent tissues by 18 h. No significant differences in intraocular bacterial growth (P ≥ 0.21) or inflammatory changes (P ≥ 0.21) were observed in eyes infected with either HBL+ or HBL? strains during the course of infection. The level of retinal responsiveness was greater in HBL? infected eyes than in HBL+-infected eyes at 6 h only (P = 0.01). These results indicate that hemolysin BL makes no essential contribution to the severe and rapid course of infection in the endophthalmitis model.
机译：蜡状芽孢杆菌是人类严重感染的罕见原因，但自相矛盾的是，它是造成眼外伤或内源性最严重感染之一的眼内炎，通常导致失明。 B的毒力。蜡状膜内膜炎历史上可归因于毒素的产生。因此，我们试图检查皮肤坏死毒素溶血素BL对B的发病机理的影响。高度易研究的眼内膜炎系统中的蜡状菌感染。玻璃体腔内注射 B的临床分离株10 2 CFU引起的感染的发病机理。用细菌学方法评估产生蜡状芽胞杆菌的溶血素BL（HBL + ）或具有此特性的同基因突变体（HBL ？），并通过裂隙灯生物显微镜，视网膜电图，组织学，和炎性细胞计数。 HBL + 和HBL ？菌株均在感染后12 h引起严重的眼内炎症反应，到12 h视网膜反应能力完全丧失。两种菌株引起的感染在18 h内就将感染传播到了相邻组织。 HBL + 感染的眼睛中，眼内细菌生长（ P ≥0.21）或炎症变化（ P ≥0.21）均无显着差异。或HBL ？菌株在感染过程中。仅在6 h时，HBL ？感染的眼睛的视网膜反应水平高于HBL + 感染的眼睛（ P = 0.01）。这些结果表明溶血素BL对眼内炎模型中的严重和快速感染过程没有实质性贡献。

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