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Aromatic Compound-Dependent Brucella suis Is Attenuated in Both Cultured Cells and Mouse Models

机译:芳香族化合物依赖的布鲁氏菌在培养的细胞和小鼠模型中均减弱。

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The aroC gene of the facultative intracellular pathogenBrucella suis was cloned and sequenced. The clonedaroC gene complements Escherichia coli andSalmonella enterica serovar Typhimurium aroCmutants. A B. suis aroC mutant was found to be unable to grow in a defined medium without aromatic compounds. The mutant was highly attenuated in tissue culture (THP1 macrophages and HeLa cells) and murine virulence models.
机译:克隆了兼性细胞内病原菌 Suuc aroC 基因并进行了测序。克隆的 aroC 基因与大肠杆菌肠炎沙门氏菌血清型鼠伤寒 aroC 突变体互补。 A B。发现suis aroC 突变体无法在没有芳香族化合物的特定培养基中生长。该突变体在组织培养(THP1巨噬细胞和HeLa细胞)和鼠毒力模型中高度减毒。

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