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首页> 外文期刊>Infection and immunity >MtuA, a Lipoprotein Receptor Antigen from Streptococcus uberis, Is Responsible for Acquisition of Manganese during Growth in Milk and Is Essential for Infection of the Lactating Bovine Mammary Gland
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MtuA, a Lipoprotein Receptor Antigen from Streptococcus uberis, Is Responsible for Acquisition of Manganese during Growth in Milk and Is Essential for Infection of the Lactating Bovine Mammary Gland

机译：MtuA是一种来自乳房链球菌的脂蛋白受体抗原，在牛奶生长过程中负责锰的采集，对于泌乳性乳腺的感染至关重要

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A mutant strain of Streptococcus uberis (AJS001) that was unable to grow in bovine milk was isolated following random insertional mutagenesis. The level of growth in milk was restored to that of the parental strain (strain 0140J) following addition of MnSO4 but not following addition of other metal ions. The mutant contained a single insertion within mtuA, a homologue of mtsA and psaA, which encode metal-binding proteins in Streptococcus pyogenes and Streptococcus pneumoniae, respectively. Strain AJS001 was unable to infect any of eight quarters on four dairy cows following intramammary challenge with 105 CFU. Bacteria were never recovered directly from milk of these animals but were detected following enrichment in Todd-Hewitt broth in three of eight milk samples obtained within 24 h of challenge. The animals showed no inflammatory response and no signs of mastitis. Three mammary quarters on two different animals simultaneously challenged with 600 CFU of the parental strain, strain 0140J, became colonized, shed high numbers of S. uberis organisms in milk, displayed a marked inflammatory response to infection, and showed overt signs of mastitis. These data indicate that mtuA was required for efficient uptake of Mn2+ during growth in bovine milk and infection of the lactating bovine mammary gland.
机译：随机插入诱变后，分离出无法在牛乳中生长的 Streptococcus uberis （AJS001）突变株。添加MnSO 4 后，牛奶的生长水平恢复到亲本菌株（菌株0140J）的水平，但添加其他金属离子后，牛奶的生长水平恢复。该突变体在 mtuA （ mtsA 和 psaA 的同系物）中包含一个插入，编码化脓性链球菌中的金属结合蛋白。 / em>和肺炎链球菌。在用10 5 CFU进行乳房内攻击后，菌株AJS001无法感染四头奶牛的八分之四。从未从这些动物的牛奶中直接回收细菌，但在攻击后24小时内获得的八份牛奶样品中，有三份在Todd-Hewitt肉汤中富集后才被发现。这些动物没有发炎反应，也没有乳腺炎的迹象。同时用600 CFU的亲本菌株0140J菌株攻击的两只不同动物的三个乳腺季度被定殖，脱落大量 S。牛奶中的乳房微生物，对感染表现出明显的炎症反应，并有明显的乳腺炎迹象。这些数据表明 mtuA 是在牛乳中生长和感染泌乳性牛乳腺期间有效摄取Mn 2 + 所必需的。

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