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Immunogenicity of Borrelia burgdorferi Arthritis-Related Protein

机译:伯氏疏螺旋体关节炎相关蛋白的免疫原性

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Immunization against arthritis-related protein (Arp) elicits antibody in mice that resolves arthritis but is not protective against challenge with Borrelia burgdorferi. In mice immunized against Arp, an unrelated 37-kDa protein (P37-42), outer surface protein A (OspA), or glutathione S-transferase (GT) and then challenged by syringe or tick, only OspA conferred protection. Passive transfer of Arp antiserum into infected SCID mice induced arthritis resolution, but antisera to P37-42, OspA, GT, or six overlapping Arp peptide fragments did not. Results suggest that the arthritis-resolving immunogenicity is specific to Arp, but the relevant epitopes may be conformational.
机译:对关节炎相关蛋白(Arp)的免疫接种会在小鼠中产生抗体,该抗体可解决关节炎,但不能抵抗伯氏疏螺旋体的攻击。在针对Arp免疫的小鼠中,不相关的37 kDa蛋白(P37-42),外表面蛋白A(OspA)或谷胱甘肽 S 转移酶(GT),然后仅通过注射器或壁虱攻击OspA赋予了保护。将Arp抗血清被动转移至感染的SCID小鼠中可诱导关节炎消退,但针对P37-42,OspA,GT或六个重叠的Arp肽片段的抗血清则不能。结果表明,解决关节炎的免疫原性对Arp具有特异性,但相关的表位可能是构象的。

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