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首页> 外文期刊>Infection and immunity >Recombinant Ochrobactrum anthropi Expressing Brucella abortus Cu,Zn Superoxide Dismutase Protects Mice against B. abortus Infection Only after Switching of Immune Responses to Th1 Type
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Recombinant Ochrobactrum anthropi Expressing Brucella abortus Cu,Zn Superoxide Dismutase Protects Mice against B. abortus Infection Only after Switching of Immune Responses to Th1 Type

机译：表达重组人流布鲁氏菌铜，锌超氧化物歧化酶的拟人ch虫仅在将免疫反应切换为Th1型后才保护小鼠免受流产布鲁氏菌感染

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The members of the genus Brucella are gram-negative, facultatively intracellular bacterial pathogens that cause brucellosis in many animal species and humans. Although live, attenuated vaccines are available to protect several animal species from the disease, there is no safe and effective vaccine for human use. Here we report that a bacterium that is closely related to Brucella species, Ochrobactrum anthropi, can be used as a vaccine vector for the delivery of Brucella antigens to mice, leading to the elicitation of protective immunity against brucellosis. Brucella abortus Cu,Zn superoxide dismutase (SOD), a protective Brucella antigen, was expressed in large amounts in O. anthropi strain 49237 by use of the broad-host-range plasmid pBBR1MCS. Neither O. anthropi strain 49237 nor the recombinant O. anthropi strain 49237SOD, expressing B. abortus Cu,Zn SOD, provided protection against virulent Brucella infection in mice. Analysis of immune responses indicated that strains 49237 and 49237SOD stimulated a mix of Th1 and Th2 type responses in the mice. After the immune response was switched to a Th1-biased response by addition of oligonucleotides containing unmethylated CpG motifs, both O. anthropi strain 49237 and the recombinant O. anthropi strain 49237SOD induced protection in mice. However, the protection conferred by strain 49237SOD was significantly better than that induced by the parental strain, 49237.
机译：布鲁氏菌属的成员是革兰氏阴性，兼性的细胞内细菌病原体，可导致许多动物和人类布鲁氏菌病。尽管可以使用减毒活疫苗来保护几种动物免受该疾病的侵害，但还没有安全，有效的人类疫苗。在这里，我们报道了一种与 Brucella 物种密切相关的细菌，即 Ochrobactrum anthropi ，可以用作递送 Brucella 的疫苗载体。小鼠抗原，导致引起针对布鲁氏菌病的保护性免疫。流产布鲁氏菌 Cu，Zn超氧化物歧化酶（SOD）是一种保护性 Brucella 抗原，在 O中大量表达。广泛宿主范围的质粒pBBR1MCS克隆人拟南芥菌株49237。都不是。 anthropi 菌株49237或重组 O。 anthropi 菌株49237SOD，表达 B。流产铜，锌超氧化物歧化酶对小鼠的强毒布鲁氏菌感染具有保护作用。免疫反应分析表明，菌株49237和49237SOD刺激了小鼠Th1和Th2型反应的混合。在通过添加含有未甲基化的CpG基序的寡核苷酸将免疫应答转换为Th1偏向的应答后，两者均为O。 anthropi 菌株49237和重组 O。 anthropi 菌株49237SOD诱导了小鼠的保护作用。但是，由菌株49237SOD赋予的保护作用明显优于由亲本菌株49237诱导的保护作用。

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