• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Conservation of Babesia bovis Small Heat Shock Protein (Hsp20) among Strains and Definition of T Helper Cell Epitopes Recognized by Cattle with Diverse Major Histocompatibility Complex Class II Haplotypes
【24h】

Conservation of Babesia bovis Small Heat Shock Protein (Hsp20) among Strains and Definition of T Helper Cell Epitopes Recognized by Cattle with Diverse Major Histocompatibility Complex Class II Haplotypes

机译：牛之间的牛肝杆菌小热休克蛋白（Hsp20）的保存和牛具有多种主要组织相容性复合体II类单倍型的T辅助细胞表位的定义

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Babesia bovis small heat shock protein (Hsp20) is recognized by CD4+ T lymphocytes from cattle that have recovered from infection and are immune to challenge. This candidate vaccine antigen is related to a protective antigen of Toxoplasma gondii, Hsp30/bag1, and both are members of the α-crystallin family of proteins that can serve as molecular chaperones. In the present study, immunofluorescence microscopy determined that Hsp20 is expressed intracellularly in all merozoites. Importantly, Hsp20 is also expressed by tick larval stages, including sporozoites, so that natural tick-transmitted infection could boost a vaccine-induced response. The predicted amino acid sequence of Hsp20 from merozoites is completely conserved among different B. bovis strains. To define the location of CD4+ T-cell epitopes for inclusion in a multiepitope peptide or minigene vaccine construct, truncated recombinant Hsp20 proteins and overlapping peptides were tested for their ability to stimulate T cells from immune cattle. Both amino-terminal (amino acids [aa] 1 to 105) and carboxy-terminal (aa 48 to 177) regions were immunogenic for the majority of cattle in the study, stimulating strong proliferation and IFN-γ production. T-cell lines from all individuals with distinct DRB3 haplotypes responded to aa 11 to 62 of Hsp20, which contained one or more immunodominant epitopes for each animal. One epitope, DEQTGLPIKS (aa 17 to 26), was identified by T-cell clones. The presence of strain-conserved T helper cell epitopes in aa 11 to 62 of the ubiquitously expressed Hsp20 that are presented by major histocompatibility complex class II molecules represented broadly in the Holstein breed supports the inclusion of this region in vaccine constructs to be tested in cattle.
机译：牛的牛肝菌小热休克蛋白（Hsp20）被牛的CD4 + T淋巴细胞识别，这些淋巴细胞已从感染中恢复过来并且免疫攻击。该候选疫苗抗原与弓形虫的保护性抗原Hsp30 / bag1相关，并且都是可作为分子伴侣的蛋白质的α-晶状体蛋白家族的成员。在本研究中，免疫荧光显微镜检查确定Hsp20在所有裂殖子的细胞内表达。重要的是，Hsp20还可以通过壁虱幼虫阶段（包括子孢子）表达，因此天然的壁虱传播感染可以促进疫苗诱导的反应。来自裂殖子的Hsp20的预测氨基酸序列在不同的 B中是完全保守的。牛栏菌株。为了确定包含在多表位肽或小基因疫苗构建物中的CD4 + T细胞表位的位置，测试了截短的重组Hsp20蛋白和重叠肽刺激免疫牛T细胞的能力。在研究中，大多数牛的氨基末端（氨基酸[aa] 1至105氨基酸）和羧基末端（aa 48至177）都是免疫原性的，从而刺激了牛的强烈增殖和IFN-γ的产生。来自具有不同 DRB3 单倍型的所有个体的T细胞系对Hsp20的第11至62位氨基酸有反应，其中Hsp20的每只动物均包含一个或多个免疫优势表位。通过T细胞克隆鉴定出一个抗原决定簇DEQTGLPIKS（aa 17至26）。广泛存在于荷斯坦犬中的主要组织相容性复合物II类分子在普遍表达的Hsp20的aa 11至62位氨基酸中存在菌株保守的T辅助细胞表位，支持将该区域包括在牛中待测试的疫苗结构中。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号