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首页> 外文期刊>Infection and immunity >Genomic Subtraction Followed by Dot Blot Screening of Streptococcus pneumoniae Clinical and Carriage Isolates Identifies Genetic Differences Associated with Strains That Cause Otitis Media
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Genomic Subtraction Followed by Dot Blot Screening of Streptococcus pneumoniae Clinical and Carriage Isolates Identifies Genetic Differences Associated with Strains That Cause Otitis Media

机译：进行基因组扣除，然后对肺炎链球菌进行临床点检和载体分离，以确定与引起中耳炎的菌株相关的遗传差异

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Streptococcus pneumoniae strains are the leading cause of bacterial otitis media, yet little is known about specific bacterial factors important for this disease. We utilized a molecular epidemiological approach involving genomic subtraction of the S. pneumoniae serogroup 19 middle ear strain 5093 against the laboratory strain R6. Resulting subtraction PCR (sPCR) products were used to screen a panel of 93 middle ear, 90 blood, 35 carriage, and 58 cerebrospinal fluid isolates from young children to identify genes found more frequently among middle ear isolates. Probe P41, similar to a hypothetical protein of Brucella melitensis, occurred among 41% of middle ear isolates and was found 2.8 (95% confidence interval [CI], 1.32 to 6.5), 3.3 (95% CI, 1.9 to 5.7), and 1.8 (95% CI, 1.1 to 3.0) times more frequently among middle ear strains than carriage, blood, or meningitis strains, respectively. sPCR fragment H10, similar to an unknown Streptococcus agalactiae protein, was present in 31% of middle ear isolates and occurred 3.6 (95% CI, 1.2 to 11.2), 2.8 (95% CI, 1.5 to 5.4), and 2.6 (95% CI, 1.2 to 5.5) times more often among middle ear isolates than carriage, blood, or meningitis strains, respectively. These studies have identified two genes of potential importance in otitis media virulence. Further studies are warranted to outline the precise role of these genes in otitis media pathogenesis.
机译：肺炎链球菌菌株是细菌性中耳炎的主要原因，但对该病重要的特定细菌因素知之甚少。我们利用了一种分子流行病学方法，其中涉及 S的基因组扣除。肺炎19血清群中耳菌株5093对实验室菌株R6。所得的减法PCR（sPCR）产品用于筛查幼儿中的93种中耳，90种血液，35种运输和58种脑脊液分离株，以鉴定在中耳分离株中发现频率更高的基因。探针P41与假单胞菌（Brucella melitensis）的一种假设蛋白相似，出现在41％的中耳分离株中，并被发现为2.8（95％置信区间[CI]，1.32至6.5），3.3（95％CI）分别比中耳炎，血液或脑膜炎菌株高1.9到5.7）和1.8（95％CI，1.1到3.0）倍。 sPCR片段H10与未知的无乳链球菌蛋白相似，存在于31％的中耳分离株中，发生率分别为3.6（95％CI，1.2至11.2），2.8（95％CI，1.5至5.4） ），在中耳分离株中的传播频率分别比运输，血液或脑膜炎毒株高2.6倍（95％CI，1.2至5.5）。这些研究已经确定了在中耳炎毒力中潜在重要的两个基因。有必要进行进一步的研究以概述这些基因在中耳炎发病机制中的确切作用。

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