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首页> 外文期刊>Infection and immunity >Interaction of Candida albicans Cell Wall Als3 Protein with Streptococcus gordonii SspB Adhesin Promotes Development of Mixed-Species Communities
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Interaction of Candida albicans Cell Wall Als3 Protein with Streptococcus gordonii SspB Adhesin Promotes Development of Mixed-Species Communities

机译：白色念珠菌细胞壁Als3蛋白与戈登链球菌SspB粘附素的相互作用促进了混合物种社区的发展。

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Candida albicans colonizes human mucosa and prosthetic surfaces associated with artificial joints, catheters, and dentures. In the oral cavity, C. albicans coexists with numerous bacterial species, and evidence suggests that bacteria may modulate fungal growth and biofilm formation. Streptococcus gordonii is found on most oral cavity surfaces and interacts with C. albicans to promote hyphal and biofilm formation. In this study, we investigated the role of the hyphal-wall protein Als3p in interactions of C. albicans with S. gordonii. Utilizing an ALS3 deletion mutant strain, it was shown that cells were not affected in initial adherence to the salivary pellicle or in hyphal formation in the planktonic phase. However, the Als3? mutant was unable to form biofilms on the salivary pellicle or deposited S. gordonii DL1 wild-type cells, and after initial adherence, als3Δ/als3Δ (ΔALS3) cells became detached concomitant with hyphal formation. In coaggregation assays, S. gordonii cells attached to, and accumulated around, hyphae formed by C. albicans wild-type cells. However, streptococci failed to attach to hyphae produced by the ΔALS3 mutant. Saccharomyces cerevisiae S150-2B cells expressing Als3p, but not control cells, supported binding of S. gordonii DL1. However, S. gordonii Δ(sspA sspB) cells deficient in production of the surface protein adhesins SspA and SspB showed >50% reduced levels of binding to S. cerevisiae expressing Als3p. Lactococcus lactis cells expressing SspB bound avidly to S. cerevisiae expressing Als3p, but not to S150-2B wild-type cells. These results show that recognition of C. albicans by S. gordonii involves Als3 protein-SspB protein interaction, defining a novel mechanism in fungal-bacterial communication.
机译：白色念珠菌定植在人类粘膜和人工关节，导管和假牙相关的义肢表面。在口腔中， C。白色念珠菌与多种细菌共存，有证据表明细菌可以调节真菌的生长和生物膜的形成。 戈登链球菌存在于大多数口腔表面，并与 C相互作用。白色念珠菌可促进菌丝和生物膜的形成。在这项研究中，我们调查了菌丝壁蛋白Als3p在 C相互作用中的作用。白色的和 S。戈多尼。利用 ALS3 缺失突变株，表明细胞在唾液膜的初始粘附或浮游期的菌丝形成方面均未受到影响。但是，Als3 ？突变体无法在唾液薄膜或沉积的 S上形成生物膜。 gordonii DL1野生型细胞，最初粘附后， als3 Δ / als3 Δ（Δ ALS3 ）细胞脱落与菌丝形成。在共聚测定中， S。戈登氏细胞附着并聚集在 C形成的菌丝周围。白色念珠菌野生型细胞。然而，链球菌未能附着在由Δ ALS3 突变体产生的菌丝上。表达Als3p的酿酒酵母 S150-2B细胞支持 S的结合，但不表达对照细胞。 gordonii DL1。但是， S。缺乏表面蛋白粘附素SspA和SspB产生的gordonii Δ（ sspA sspB ）细胞显示与 S的结合水平降低了50％以上。表达Als3p的啤酒酵母。表达SspB的乳酸乳球菌细胞与 S强烈结合。啤酒酵母表达Als3p，但不表达S150-2B野生型细胞。这些结果表明对 C的识别。白色的 gordonii 涉及Als3蛋白与SspB蛋白的相互作用，为真菌与细菌的相互作用定义了一种新的机制。

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