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首页> 外文期刊>Infection and immunity >Two Atypical Enteropathogenic Escherichia coli Strains Induce the Production of Secreted and Membrane-Bound Mucins To Benefit Their Own Growth at the Apical Surface of Human Mucin-Secreting Intestinal HT29-MTX Cells
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Two Atypical Enteropathogenic Escherichia coli Strains Induce the Production of Secreted and Membrane-Bound Mucins To Benefit Their Own Growth at the Apical Surface of Human Mucin-Secreting Intestinal HT29-MTX Cells

机译：两种非典型肠致病性大肠杆菌菌株诱导分泌和膜结合粘蛋白的产生，以使其在人粘蛋白分泌肠HT29-MTX细胞顶表面的生长受益。

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摘要

In rabbit ligated ileal loops, two atypical enteropathogenic Escherichia coli (aEPEC) strains, 3991-1 and 0421-1, intimately associated with the cell membrane, forming the characteristic EPEC attachment and effacement lesion of the brush border, induced a mucous hypersecretion, whereas typical EPEC (tEPEC) strain E2348/69 did not. Using cultured human mucin-secreting intestinal HT29-MTX cells, we demonstrate that apically aEPEC infection is followed by increased production of secreted MUC2 and MUC5AC mucins and membrane-bound MUC3 and MUC4 mucins. The transcription of the MUC5AC and MUC4 genes was transiently upregulated after aEPEC infection. We provide evidence that the apically adhering aEPEC cells exploit the mucins' increased production since they grew in the presence of membrane-bound mucins, whereas tEPEC did not. The data described herein report a putative new virulence phenomenon in aEPEC.
机译：在兔子结扎的回肠环中，两种非典型的肠致病性大肠埃希氏大肠杆菌（aEPEC）菌株3991-1和0421-1与细胞膜紧密相关，形成了特征性的EPEC附着和刷缘的脱落病灶诱导粘液分泌过多，而典型的EPEC（tEPEC）菌株E2348 / 69没有。使用培养的人类分泌粘蛋白的肠道HT29-MTX细胞，我们证明了根尖aEPEC感染后分泌的MUC2和MUC5AC粘蛋白以及膜结合MUC3和MUC4粘蛋白的产量增加。在aEPEC感染后， MUC5AC 和 MUC4 基因的转录瞬时上调。我们提供的证据表明，顶端粘附的aEPEC细胞利用了粘蛋白的增加的产量，因为它们在存在膜结合粘蛋白的情况下生长，而tEPEC则没有。本文描述的数据报告了aEPEC中的一种新的毒力现象。

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