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首页> 外文期刊>Infection and immunity >UpaH Is a Newly Identified Autotransporter Protein That Contributes to Biofilm Formation and Bladder Colonization by Uropathogenic Escherichia coli CFT073
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UpaH Is a Newly Identified Autotransporter Protein That Contributes to Biofilm Formation and Bladder Colonization by Uropathogenic Escherichia coli CFT073

机译：UpaH是一种新鉴定的自转运蛋白，可通过致病性大肠杆菌CFT073促进生物膜形成和膀胱定植。

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Escherichia coli is the primary cause of urinary tract infection (UTI) in the developed world. The major factors associated with virulence of uropathogenic E. coli (UPEC) are fimbrial adhesins, which mediate specific attachment to host receptors and trigger innate host responses. Another group of adhesins is represented by the autotransporter (AT) subgroup of proteins. In this study, we identified a new AT-encoding gene, termed upaH, present in a 6.5-kb unannotated intergenic region in the genome of the prototypic UPEC strain CFT073. Cloning and sequencing of the upaH gene from CFT073 revealed an intact 8.535-kb coding region, contrary to the published genome sequence. The upaH gene was widely distributed among a large collection of UPEC isolates as well as the E. coli Reference (ECOR) strain collection. Bioinformatic analyses suggest β-helix as the predominant structure in the large N-terminal passenger (α) domain and a 12-strand β-barrel for the C-terminal β-domain of UpaH. We demonstrated that UpaH is expressed at the cell surface of CFT073 and promotes biofilm formation. In the mouse UTI model, deletion of the upaH gene in CFT073 and in two other UPEC strains did not significantly affect colonization of the bladder in single-challenge experiments. However, in competitive colonization experiments, CFT073 significantly outcompeted its upaH isogenic mutant strain in urine and the bladder.
机译：大肠杆菌是发达国家的尿路感染（UTI）的主要原因。与尿毒症 E毒力相关的主要因素。大肠杆菌（UPEC）是纤维黏附素，可介导宿主受体的特异性附着并触发先天宿主应答。另一组粘附素由蛋白质的自转运子（AT）亚组代表。在这项研究中，我们确定了一个新的AT编码基因，称为 upaH ，它存在于原型UPEC菌株CFT073基因组的6.5 KB无注释基因间区域中。来自CFT073的 upaH 基因的克隆和测序揭示了完整的8.535-kb编码区，与已公开的基因组序列相反。 upaH 基因广泛分布在许多UPEC分离株以及 E中。大肠杆菌参考（ECOR）菌株集合。生物信息学分析表明，β-螺旋是大N末端过客（α）结构域的主要结构，而12链β-桶则是UpaH的C末端β结构域的结构。我们证明了UpaH在CFT073的细胞表面表达并促进生物膜形成。在小鼠UTI模型中，在单攻击实验中，CFT073和其他两个UPEC菌株中 upaH 基因的缺失并未显着影响膀胱的定植。但是，在竞争性定植实验中，CFT073在尿液和膀胱中的竞争能力明显优于其 upaH 同基因突变株。

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