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首页> 外文期刊>Infection and immunity >Transcutaneous Immunization Using Colonization Factor and Heat-Labile Enterotoxin Induces Correlates of Protective Immunity for Enterotoxigenic Escherichia coli
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Transcutaneous Immunization Using Colonization Factor and Heat-Labile Enterotoxin Induces Correlates of Protective Immunity for Enterotoxigenic Escherichia coli

机译：使用定居因子和不耐热肠毒素的经皮免疫诱导对产肠毒素的大肠杆菌的保护性免疫的相关性。

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Enterotoxigenic Escherichia coli (ETEC) diarrheal disease is a worldwide problem that may be addressed by transcutaneous delivery of a vaccine. In several human settings, protective immunity has been associated with immune responses to E. coli colonization factors and to the heat-labile toxin that induces the diarrhea. In this set of animal studies, transcutaneous immunization (TCI) using recombinant colonization factor CS6 and cholera toxin (CT) or heat-labile enterotoxin (LT) as the adjuvant induced immunoglobulin G (IgG) and IgA anti-CS6 responses in sera and stools and antibody responses that recognized CS6 antigen in its native configuration. The antitoxin immunity induced by TCI was also shown to protect against enteric toxin challenge. Although immunization with LT via the skin induced mucosal secretory IgA responses to LT, protection could also be achieved by intravenous injection of the immune sera. Finally, a malaria vaccine antigen, merzoite surface protein 142 administered with CT as the adjuvant, induced both merzoite surface protein antibodies and T-cell responses while conferring protective antitoxin immunity, suggesting that both antiparasitic activity and antidiarrheal activity can be obtained with a single vaccine formulation. Overall, our results demonstrate that relevant colonization factor and antitoxin immunity can be induced by TCI and suggest that an ETEC traveler's diarrhea vaccine could be delivered by using a patch.
机译：肠毒素性大肠杆菌（ETEC）腹泻病是一个世界性的问题，可以通过经皮输送疫苗来解决。在几种人类环境中，保护性免疫已与对 E的免疫反应相关。大肠菌群定殖因子和引起腹泻的不耐热毒素。在这组动物研究中，使用重组定殖因子CS6和霍乱毒素（CT）或热不稳定肠毒素（LT）作为佐剂在血清和粪便中诱导的免疫球蛋白G（IgG）和IgA抗CS6反应进行经皮免疫（TCI）和识别CS6抗原的天然构型的抗体反应。还显示了TCI诱导的抗毒素免疫力可抵抗肠毒素攻击。尽管通过皮肤免疫接种LT诱导了对LT的粘膜分泌性IgA反应，但也可以通过静脉注射免疫血清来实现保护。最后，以CT为佐剂的疟疾疫苗抗原，裂殖子表面蛋白1 42 诱导了裂殖子表面蛋白抗体和T细胞反应，同时赋予了保护性抗毒素免疫力，表明抗寄生虫活性和止泻作用可以用单一疫苗制剂获得活性。总体而言，我们的结果表明，TCI可以诱导相关的定居因子和抗毒素免疫力，并表明可以通过使用贴片来运送ETEC旅行者的腹泻疫苗。

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