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首页> 外文期刊>Infection and immunity >Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression
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Role of Glutathione Metabolism of Treponema denticola in Bacterial Growth and Virulence Expression

机译：密闭螺旋体谷胱甘肽代谢在细菌生长和毒力表达中的作用

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Hydrogen sulfide (H2S) is a major metabolic end product detected in deep periodontal pockets that is produced by resident periodontopathic microbiota associated with the progression of periodontitis. Treponema denticola, a member of the subgingival biofilm at disease sites, produces cystalysin, an enzyme that catabolizes cysteine, releasing H2S. The metabolic pathway leading to H2S formation in periodontal pockets has not been determined. We used a variety of thiol compounds as substrates for T. denticola to produce H2S. Our results indicate that glutathione, a readily available thiol source in periodontal pockets, is a suitable substrate for H2S production by this microorganism. In addition to H2S, glutamate, glycine, ammonia, and pyruvate were metabolic end products of metabolism of glutathione. Cysteinyl glycine (Cys-Gly) was also catabolized by the bacteria, yielding glycine, H2S, ammonia, and pyruvate. However, purified cystalysin could not catalyze glutathione and Cys-Gly degradation in vitro. Moreover, the enzymatic activity(ies) in T. denticola responsible for glutathione breakdown was inactivated by trypsin or proteinase K, by heating (56°C) and freezing (?20°C), by sonication, and by exposure to Nα-p-tosyl-l-lysine chloromethyl ketone (TLCK). These treatments had no effect on degradation of cysteine by the purified enzyme. In this study we delineated an enzymatic pathway for glutathione metabolism in the oral spirochete T. denticola; our results suggest that glutathione metabolism plays a role in bacterial nutrition and potential virulence expression.
机译：硫化氢（H 2 S）是在深部牙周袋中检测到的主要代谢终产物，其是由与牙周炎发展相关的常驻牙周病菌群产生的。致病部位龈下生物膜的成员 Treponema denticola 会产生半胱氨酸蛋白酶，一种分解半胱氨酸的酶，释放H 2 S。尚未确定导致牙周袋中H 2 S形成的代谢途径。我们使用了多种硫醇化合物作为 T的底物。树生以产生H 2 S。我们的结果表明，谷胱甘肽是一种可在牙周袋中容易获得的硫醇来源，是该微生物生产H 2 S的合适底物。除了H 2 S，谷氨酸，甘氨酸，氨和丙酮酸都是谷胱甘肽代谢的代谢终产物。半胱氨酸甘氨酸（Cys-Gly）也被细菌分解代谢，产生甘氨酸，H 2 S，氨和丙酮酸。然而，纯化的胱溶酶不能在体外催化谷胱甘肽和Cys-Gly的降解。此外， T中的酶活性。胰蛋白酶或蛋白酶K，加热（56°C）和冷冻（？20°C），超声处理以及暴露于 N α- p -甲苯磺酰基-1-赖氨酸氯甲基酮（TLCK）。这些处理对纯化的酶对半胱氨酸的降解没有影响。在这项研究中，我们描述了口服螺旋体 T中谷胱甘肽代谢的酶促途径。树突藻;我们的结果表明，谷胱甘肽代谢在细菌营养和潜在毒力表达中起作用。

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