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首页> 外文期刊>Infection and immunity >Sat, the Secreted Autotransporter Toxin of Uropathogenic Escherichia coli, Is a Vacuolating Cytotoxin for Bladder and Kidney Epithelial Cells
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Sat, the Secreted Autotransporter Toxin of Uropathogenic Escherichia coli, Is a Vacuolating Cytotoxin for Bladder and Kidney Epithelial Cells

机译：星期六，尿毒症性大肠杆菌的分泌的自转运毒素，是膀胱和肾脏上皮细胞的一种真空细胞毒素。

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The secreted autotransporter toxin (Sat) of uropathogenic Escherichia coli exhibits cytopathic activity upon incubation with HEp-2 cells. We further investigated the effects of Sat on cell lines more relevant to the urinary tract, namely, those derived from bladder and kidney epithelium. Sat elicited elongation of cells and apparent loosening of cellular junctions upon incubation with Vero kidney cells. Additionally, incubation with Sat triggered significant vacuolation within the cytoplasm of both human bladder (CRL-1749) and kidney (CRL-1573) cell lines. This activity has been associated with only a few other known toxins. Following transurethral infection of CBA mice with a sat mutant, no reduction of CFU in urine, bladder, or kidney tissue was seen compared to that in mice infected with wild-type E. coli CFT073. However, significant histological changes were observed within the kidneys of mice infected with wild-type E. coli CFT073, including dissolution of the glomerular membrane and vacuolation of proximal tubule cells. Such damage was not observed in kidney sections of mice infected with a Sat-deficient mutant. These results indicate that Sat, a vacuolating cytotoxin expressed by uropathogenic E. coli CFT073, elicits defined damage to kidney epithelium during upper urinary tract infection and thus contributes to pathogenesis of urinary tract infection.
机译：泌尿道致病性大肠杆菌分泌的自转运毒素（Sat）在与HEp-2细胞孵育后表现出细胞病变活性。我们进一步研究了Sat对与泌尿道更为相关的细胞系的影响，这些细胞系来自膀胱和肾脏上皮细胞。与Vero肾细胞孵育后，Sat引起细胞伸长和细胞连接明显松弛。此外，与Sat孵育会触发人膀胱（CRL-1749）和肾（CRL-1573）细胞系细胞质内的明显空泡。该活性仅与其他几种已知毒素有关。与 E野生型小鼠相比，经 sat 突变体经CBA小鼠经尿道感染后，尿，膀胱或肾脏组织中的CFU未见降低。大肠杆菌CFT073。但是，在野生型 E感染的小鼠肾脏中观察到了明显的组织学变化。大肠杆菌CFT073，包括肾小球膜的溶解和近端小管细胞的空泡化。在感染了Sat缺陷突变体的小鼠的肾脏切片中未观察到这种损伤。这些结果表明，Sat是一种由尿路致病性 E表达的空泡细胞毒素。大肠杆菌CFT073在上尿路感染期间引起明显的肾上皮损害，从而促进了尿路感染的发病机理。

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