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首页> 外文期刊>Infection and immunity >Host Adaptation of Pigeon Isolates of Salmonella enterica subsp. enterica Serovar Typhimurium Variant Copenhagen Phage Type 99 Is Associated with Enhanced Macrophage Cytotoxicity
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Host Adaptation of Pigeon Isolates of Salmonella enterica subsp. enterica Serovar Typhimurium Variant Copenhagen Phage Type 99 Is Associated with Enhanced Macrophage Cytotoxicity

机译：肠道沙门氏菌亚种鸽分离株的宿主适应。肠杆菌血清型鼠伤寒变种哥本哈根噬菌体99型与增强的巨噬细胞细胞毒性有关。

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Phage type 99 of Salmonella enterica subsp. enterica serovar Typhimurium variant Copenhagen strains isolated from pigeons were examined for the presence of genotypic and phenotypic characteristics. The pulsed-field gel electrophoresis patterns obtained with XbaI and BlnI from 38 pigeon strains were compared with those obtained from 89 porcine, poultry, and human strains of variant Copenhagen. Identical patterns with XbaI and four closely related patterns with BlnI were obtained with the pigeon strains, whereas 16 XbaI patterns were found with the other strains. The XbaI patterns of the pigeon strains showed a low genetic similarity to the patterns of the porcine, poultry, and human strains and invariably showed a low-molecular-weight band that was absent in the majority of the other strains. The virulence genes shdA, spvR, pefA, sopE, and spvB were uniformly present in six pigeon isolates representing the genetic diversity found with BlnI. These six pigeon-derived strains were highly cytotoxic for pigeon macrophages compared to three porcine strains. After experimental infection of pigeons with a pigeon strain, clinical symptoms, fecal shedding, and colonization of internal organs were more pronounced than those after infection with a porcine strain. These data suggest that the phage type 99 strains used in this study are highly adapted to pigeons and should be classified as a host-restricted lineage of the serovar Typhimurium.
机译：沙门氏菌亚种的99型噬菌体。从鸽子中分离出的 enterica 鼠伤寒沙门氏菌变种哥本哈根品系存在基因型和表型特征。比较了从38株鸽系的 Xba I和 Bln I获得的脉冲场凝胶电泳图谱，以及从89种猪，禽和人的哥本哈根变种获得的脉冲场凝胶电泳图谱。用鸽系获得了与 Xba I相同的模式和与 Bln I紧密相关的四个模式，而与之相比，发现了16个 Xba I模式其他菌株。鸽子品系的 Xba I型与猪，家禽和人品系的遗传相似性较低，并且在大多数猪中均不存在低分子量谱带。其他菌株。毒力基因 shdA ， spvR ， pefA ， sopE 和 spvB 是一致的出现在六个代表 Bln I的遗传多样性的鸽子分离物中。与三种猪株相比，这六种鸽源菌株对鸽子巨噬细胞具有高度的细胞毒性。用鸽子株对鸽子进行实验性感染后，其临床症状，粪便脱落和内部器官定植要比感染猪株后更为明显。这些数据表明，在这项研究中使用的99型噬菌体菌株非常适合鸽子，应该被归类为鼠伤寒沙门氏菌的宿主限制性谱系。

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