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首页> 外文期刊>Infection and immunity >Molecular Heterogeneity of Ehrlichia chaffeensis Isolates Determined by Sequence Analysis of the 28-Kilodalton Outer Membrane Protein Genes and Other Regions of the Genome
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Molecular Heterogeneity of Ehrlichia chaffeensis Isolates Determined by Sequence Analysis of the 28-Kilodalton Outer Membrane Protein Genes and Other Regions of the Genome

机译：通过28-Kilodalton外膜蛋白基因和该基因组其他区域的序列分析确定了恰菲埃里希氏菌分离物的分子异质性

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摘要

Ehrlichia chaffeensis, a tick-transmitted rickettsial agent, is responsible for human monocytic ehrlichiosis (HME). In this study, we genetically mapped 10 isolates obtained from HME patients. Sequence analysis of the 28-kDa outer membrane protein (OMP) multigene locus spanning 6 of the 22 tandemly arranged genes identified three distinct genetic groups with shared homology among isolates within each group. Isolates in Groups I and III contained six genes each, while Group II isolates had a gene deletion. There were two regions on the locus where novel gene deletion or insertion mutations occurred, resulting in the net loss of one gene in Group II isolates. Numerous nucleotide differences among genes in isolates of each group also were detected. The shared homology among isolates in each group for the 28-kDa OMP locus suggests the derivation of clonal lineages. Transcription and translation analysis of the locus revealed differences in the expressed genes of different group isolates. Analysis of the 120-kDa OMP gene and variable-length PCR target gene showed size variations resulting from loss or gain of long, direct repeats within the protein coding sequences. To our knowledge this is the first study that looked at several regions of the genome simultaneously, and we provide the first evidence of heterogeneity resulting from gene deletion and insertion mutations in the E. chaffeensis genome. Diversity in different genomic regions could be the result of a selection process or of independently evolved genes.
机译：em传播的立克次体菌 Ehrlichia chaffeensis 负责人类单核细胞埃希氏菌病（HME）。在这项研究中，我们对从HME患者获得的10个分离株进行了基因定位。跨越22个串联排列的基因中的6个的28 kDa外膜蛋白（OMP）多基因位点的序列分析确定了三个不同的基因组，每个组中的分离株之间具有相同的同源性。第一和第三组的分离株每个包含六个基因，而第二组的分离株具有基因缺失。在基因座上有两个区域，其中发生新的基因缺失或插入突变，导致II组分离物中一个基因的净损失。每组分离物中的基因之间也检测到许多核苷酸差异。每一组中28 kDa OMP位点的分离株之间具有相同的同源性，表明克隆谱系的衍生。基因座的转录和翻译分析揭示了不同组分离株表达基因的差异。对120 kDa OMP基因和可变长度PCR靶基因的分析显示，大小差异是由于蛋白质编码序列中长而直接的重复序列的丢失或获得所致。据我们所知，这是第一项同时研究基因组多个区域的研究，并且我们提供了由 E基因缺失和插入突变导致异质性的第一个证据。 chaffeensis 基因组。不同基因组区域的多样性可能是选择过程或独立进化基因的结果。

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