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首页> 外文期刊>Infection and immunity >A K+ Uptake Protein, TrkA, Is Required for Serum, Protamine, and Polymyxin B Resistance in Vibrio vulnificus
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A K+ Uptake Protein, TrkA, Is Required for Serum, Protamine, and Polymyxin B Resistance in Vibrio vulnificus

机译：创伤弧菌的血清，鱼精蛋白和多粘菌素B抗性需要K +摄取蛋白TrkA

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摘要

Vibrio vulnificus, a highly virulent marine bacterium, is the causative agent of both serious wound infections and fatal septicemia in many areas of the world. To identify the genes required for resistance to human serum, we constructed a library of transposon mutants of V. vulnificus and screened them for hypersensitivity to human serum. Here we report that one of the isolated serum-susceptible mutants had a mutation in an open reading frame identified as trkA, a gene encoding an amino acid sequence showing high identity to that of TrkA of Vibrio alginolyticus, a protein required for the uptake of potassium. A trkA isogenic mutant was constructed via insertional inactivation, and it was significantly more easily killed by human serum, protamine, or polymyxin B than was the wild type. At K+ concentrations of 1 to 20 mM, this isogenic mutant showed attenuated growth compared to the wild-type strain. In addition, infection experiments demonstrated virulence attenuation when this mutant was administered intraperitoneally or subcutaneously to both normal and iron-treated mice, indicating that TrkA may modulate the transport of potassium and resistance to host innate defenses and that it is important for virulence in mice.
机译：剧毒弧菌是一种高毒性海洋细菌，是世界许多地区严重伤口感染和致命败血症的病原体。为了鉴定抗人血清所需的基因，我们构建了 V转座子突变体文库。并筛选它们对人血清的超敏反应。在这里，我们报告说，其中一个分离的血清易感突变体在一个开放阅读框中具有一个突变，被识别为 trkA ，该基因编码的氨基酸序列与弧菌的TrkA具有高度同一性alginolyticus ，一种吸收钾的蛋白质。一个 trkA 同基因突变体是通过插入失活构建的，与野生型相比，它更容易被人血清，鱼精蛋白或多粘菌素B杀死。在K + 浓度为1至20 mM时，该同基因突变体与野生型菌株相比显示出减毒的生长。此外，感染实验证明，当将该突变体经腹膜内或皮下给予正常和铁处理的小鼠时，毒力减弱，表明TrkA可能调节钾的转运和对宿主先天防御的抵抗力，这对于小鼠中的毒力很重要。

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