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首页> 外文期刊>Infection and immunity >OI-57, a Genomic Island of Escherichia coli O157, Is Present in Other Seropathotypes of Shiga Toxin-Producing E. coli Associated with Severe Human Disease
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OI-57, a Genomic Island of Escherichia coli O157, Is Present in Other Seropathotypes of Shiga Toxin-Producing E. coli Associated with Severe Human Disease

机译：OI-57，大肠杆菌O157的基因组岛，存在于与严重人类疾病相关的产志贺毒素的其他血清型的大肠杆菌中

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Strains of Shiga toxin-producing Escherichia coli (STEC) are a heterogeneous E. coli group that may cause severe disease in humans. STEC have been categorized into seropathotypes (SPTs) based on their phenotypic and molecular characteristics and the clinical features of the associated diseases. SPTs range from A to E, according to a decreasing rank of pathogenicity. To define the virulence gene asset (“virulome”) characterizing the highly pathogenic SPTs, we used microarray hybridization to compare the whole genomes of STEC belonging to SPTs B, C, and D with that of STEC O157 (SPT A). The presence of the open reading frames (ORFs) associated with SPTs A and B was subsequently investigated by PCR in a larger panel of STEC and in other E. coli strains. A genomic island termed OI-57 was present in SPTs A and B but not in the other SPTs. OI-57 harbors the putative virulence gene adfO, encoding a factor enhancing the adhesivity of STEC O157, and ckf, encoding a putative killing factor for the bacterial cell. PCR analyses showed that OI-57 was present in its entirety in the majority of the STEC genomes examined, indicating that it represents a stable acquisition of the positive clonal lineages. OI-57 was also present in a high proportion of the human enteropathogenic E. coli genomes assayed, suggesting that it could be involved in the attaching-and-effacing colonization of the intestinal mucosa. In conclusion, OI-57 appears to be part of the virulome of pathogenic STEC and further studies are needed to elucidate its role in the pathogenesis of STEC infections.
机译：产志贺毒素的大肠杆菌（STEC）菌株是异源的 E。可能导致人类严重疾病的大肠菌群。根据STEC的表型和分子特征以及相关疾病的临床特征，已将其分类为血清型（SPT）。根据致病程度的降低，SPT的范围从A到E。为了定义表征高致病性SPT的毒力基因资产（“病毒”），我们使用微阵列杂交比较了属于SPT B，C和D的STEC的全基因组与STEC O157（SPT A）的全基因组。随后在较大的STEC和其他Eem中通过PCR研究了与SPT A和B相关的开放阅读框（ORF）的存在。大肠杆菌菌株。在SPT A和B中存在一个称为OI-57的基因岛，但在其他SPT中却没有。 OI-57包含推定毒力基因 adfO ，它编码一个增强STEC O157粘附力的因子，而 ckf ，它编码该细菌细胞的假定杀伤因子。 PCR分析表明，OI-57完整存在于大多数STEC基因组中，表明它代表了阳性克隆谱系的稳定获取。 OI-57也​​存在于人类肠道致病菌 E中的比例很高。对大肠杆菌基因组进行了测定，表明它可能参与了肠道粘膜的附着和脱落定殖。总之，OI-57似乎是致病性STEC病毒学的一部分，需要进一步研究以阐明其在STEC感染的发病机理中的作用。

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