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Identification and Characterization of Two Bordetella avium Gene Products Required for Hemagglutination

机译：血凝所需的两种博德氏杆菌鸟基因产品的鉴定和表征

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Bordetella avium causes bordetellosis in birds, a disease similar to whooping cough caused by Bordetella pertussis in children. B. avium agglutinates guinea pig erythrocytes via an unknown mechanism. Loss of hemagglutination ability results in attenuation. We report the use of transposon mutagenesis to identify two genes required for hemagglutination. The genes (hagA and hagB) were adjacent and divergently oriented and had no orthologs in the genomes of other Bordetella species. Construction of in-frame, unmarked mutations in each gene allowed examination of the role of each in conferring erythrocyte agglutination, explanted tracheal cell adherence, and turkey poult tracheal colonization. In all of the in vitro and in vivo assays, the requirement for the trans-acting products of hagA and hagB (HagA and HagB) was readily shown. Western blotting, using antibodies to purified HagA and HagB, revealed proteins of the predicted sizes of HagA and HagB in an outer membrane-enriched fraction. Antiserum to HagB, but not HagA, blocked B. avium erythrocyte agglutination and explanted turkey tracheal ring binding. Bioinformatic analysis indicated the similarity of HagA and HagB to several two-component secretory apparatuses in which one product facilitates the exposition of the other. HagB has the potential to serve as a useful immunogen to protect turkeys against colonization and subsequent disease.
机译：鸟粪贝氏杆菌在鸟类中引起波尔多菌病，这种疾病类似于儿童百日咳杆菌百日咳引起的百日咳。 B。鸟毒素通过未知的机制凝集豚鼠的红细胞。血凝能力的丧失导致衰减。我们报告使用转座子诱变来识别血凝所需的两个基因。基因（ hagA 和 hagB ）相邻且趋异，在其他 Bordetella 物种的基因组中没有直系同源基因。在每个基因中构建符合读框的未标记突变，可以检查每个基因在赋予红细胞凝集，移出气管细胞粘附以及火鸡家禽气管定植中的作用。在所有体外和体内分析中，对 hagA 和 hagA 的反式作用产物的要求 hagB （HagA和HagB）很容易显示。使用针对纯化的HagA和HagB的抗体进行的蛋白质印迹显示，在富含外膜的部分中，具有预测大小的HagA和HagB的蛋白质。对HagB而非HagA的抗血清阻止了 B。鸟红细胞凝集和外植火鸡气管环结合。生物信息学分析表明，HagA和HagB与几种两组分分泌装置的相似性，其中一种产物促进另一种产物的暴露。 HagB有潜力作为有用的免疫原来保护火鸡免受定居和随后的疾病侵袭。

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