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首页> 外文期刊>Infection and immunity >Burkholderia cenocepacia ShvR-Regulated Genes That Influence Colony Morphology, Biofilm Formation, and Virulence
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Burkholderia cenocepacia ShvR-Regulated Genes That Influence Colony Morphology, Biofilm Formation, and Virulence

机译：Burkholderia cenocepacia ShvR调控的基因，影响菌落形态，生物膜形成和毒力

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Burkholderia cenocepacia is an opportunistic pathogen that primarily infects cystic fibrosis (CF) patients. Previously, we reported that ShvR, a LysR regulator, influences colony morphology, virulence, and biofilm formation and regulates the expression of an adjacent 24-kb genomic region encoding 24 genes. In this study, we report the functional characterization of selected genes in this region. A Tn5 mutant with shiny colony morphology was identified with a polar mutation in BCAS0208, predicted to encode an acyl-coenzyme A dehydrogenase. Mutagenesis of BCAS0208 and complementation analyses revealed that BCAS0208 is required for rough colony morphology, biofilm formation, and virulence on alfalfa seedlings. It was not possible to complement with BCAS0208 containing a mutation in the catalytic site. BCAS0201, encoding a putative flavin adenine dinucleotide (FAD)-dependent oxidoreductase, and BCAS0207, encoding a putative citrate synthase, do not influence colony morphology but are required for optimum levels of biofilm formation and virulence. Both BCAS0208 and BCAS0201 contribute to pellicle formation, although individual mutations in each of these genes had no appreciable effect on pellicle formation. A mutant with a polar insertion in BCAS0208 was significantly less virulent in a rat model of chronic lung infection as well as in the alfalfa model. Genes in this region were shown to influence utilization of branched-chain fatty acids, tricarboxylic acid cycle substrates, l-arabinose, and branched-chain amino acids. Together, our data show that the ShvR-regulated genes BCAS0208 to BCAS0201 are required for the rough colony morphotype, biofilm and pellicle formation, and virulence in B. cenocepacia.
机译：Burkholderia cenocepacia是一种机会病原体，主要感染囊性纤维化（CF）患者。以前，我们报道过，LysR调节器ShvR影响菌落形态，毒力和生物膜形成，并调节编码24个基因的相邻24 kb基因组区域的表达。在这项研究中，我们报告了该区域中选定基因的功能表征。在BCAS0208中鉴定出一个带有闪亮菌落形态的Tn 5 突变体，该突变体带有一个极性突变，预计可编码一个酰基辅酶A脱氢酶。 BCAS0208的诱变和互补分析表明，苜蓿幼苗的粗糙菌落形态，生物膜形成和毒力需要BCAS0208。不可能与在催化位点包含突变的BCAS0208互补。编码假定的黄素腺嘌呤二核苷酸（FAD）依赖性氧化还原酶的BCAS0201和编码假定的柠檬酸合酶的BCAS0207不会影响菌落的形态，但对于生物膜形成和毒力的最佳水平是必需的。 BCAS0208和BCAS0201均有助于防护膜的形成，尽管这些基因中的每个基因的个体突变对防护膜的形成均无明显影响。在慢性肺部感染的大鼠模型以及苜蓿模型中，在BCAS0208中具有极性插入的突变体的毒性均明显较低。已显示该区域中的基因会影响支链脂肪酸，三羧酸循环底物，1-阿拉伯糖和支链氨基酸的利用率。在一起，我们的数据表明，ShvR调控的基因BCAS0208至BCAS0201是粗糙菌落形态，生物膜和表膜形成以及毒力在B. cenocepacia中的必需条件。

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