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首页> 外文期刊>Infection and immunity >Branching Network of Proteinaceous Filaments within the Parasitophorous Vacuole of Encephalitozoon cuniculi and Encephalitozoon hellem
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Branching Network of Proteinaceous Filaments within the Parasitophorous Vacuole of Encephalitozoon cuniculi and Encephalitozoon hellem

机译：头状脑病和脑性乙脑的寄生虫脉状蛋白丝的分支网络

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The microsporidia are a diverse phylum of obligate intracellular parasites that infect all major animal groups and have been recognized as emerging human pathogens for which few chemotherapeutic options currently exist. These organisms infect every tissue and organ system, causing significant pathology, especially in immune-compromised populations. The microsporidian spore employs a unique infection strategy in which its contents are delivered into a host cell via the polar tube, an organelle that lies coiled within the resting spore but erupts with a force sufficient to pierce the plasma membrane of its host cell. Using biochemical and molecular approaches, we have previously identified components of the polar tube and spore wall of the Encephalitozoonidae. In this study, we employed a shotgun proteomic strategy to identify novel structural components of these organelles in Encephalitozoon cuniculi. As a result, a new component of the E. cuniculi developing spore wall was identified. Surprisingly, using the same approach, a heretofore undescribed filamentous network within the lumen of the parasitophorous vacuole was discovered. This network was also present in the parasitophorous vacuole of Encephalitozoon hellem. Thus, in addition to further elucidating the molecular composition of seminal organelles and revealing novel diagnostic and therapeutic targets, proteomic analysis-driven approaches exploring the spore may also uncover unknown facets of microsporidian biology.
机译：微孢子虫是感染所有主要动物群的专性细胞内寄生虫的多样门，并且已经被认为是新兴的人类病原体，目前对其化学疗法的选择很少。这些生物感染每个组织和器官系统，导致严重的病理变化，尤其是在免疫功能低下的人群中。微孢子虫孢子采用独特的感染策略，其中其内含物通过极管传递到宿主细胞中，该细胞器盘绕在静止的孢子中，但爆发力足以刺穿其宿主细胞的质膜。使用生化和分子方法，我们先前已经确定了极脑虫科的极管和孢子壁的成分。在这项研究中，我们采用了shot弹枪蛋白质组学策略，来识别 Encephalitozoon cuniculi 中这些细胞器的新型结构成分。结果，是 E的新组成部分。鉴定到了孢子壁发育的孢子。出乎意料的是，使用相同的方法，发现了寄生虫空泡内腔内迄今未描述的丝状网络。该网络也存在于 Encephalitozoon hellem 的寄生虫液泡中。因此，除了进一步阐明精子细胞器的分子组成并揭示新的诊断和治疗靶标外，蛋白质组学分析驱动的探索孢子的方法还可能发现微孢子虫生物学的未知方面。

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