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Critical Role of LuxS in the Virulence of Campylobacter jejuni in a Guinea Pig Model of Abortion

机译：LuxS在豚鼠流产模型中空肠弯曲杆菌毒力中的关键作用

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Previous studies on Campylobacter jejuni have demonstrated the role of LuxS in motility, cytolethal distending toxin production, agglutination, and intestinal colonization; however, its direct involvement in virulence has not been reported. In this study, we demonstrate a direct role of luxS in the virulence of C. jejuni in two different animal hosts. The IA3902 strain, a highly virulent sheep abortion strain recently described by our laboratory, along with its isogenic luxS mutant and luxS complement strains, was inoculated by the oral route into both a pregnant guinea pig virulence model and a chicken colonization model. In both cases, the IA3902 luxS mutant demonstrated a complete loss of ability to colonize the intestinal tract. In the pregnant model, the mutant also failed to induce abortion, while the wild-type strain was highly abortifacient. Genetic complementation of the luxS gene fully restored the virulent phenotype in both models. Interestingly, when the organism was inoculated into guinea pigs by the intraperitoneal route, no difference in virulence (abortion induction) was observed between the luxS mutant and the wild-type strain, suggesting that the defect in virulence following oral inoculation is likely associated with a defect in colonization and/or translocation of the organism out of the intestine. These studies provide the first direct evidence that LuxS plays an important role in the virulence of C. jejuni using an in vivo model of natural disease.
机译：以前对空肠弯曲菌的研究表明，LuxS在运动性，细胞致死性扩展毒素产生，凝集和肠道菌落形成中起着作用。然而，尚未报道其直接参与毒力。在这项研究中，我们证明了luxS在两种不同动物宿主中空肠弯曲杆菌毒力中的直接作用。 IA3902菌株是我们实验室最近描述的一种高毒力羊流产菌株，以及它的同基因luxS突变体和luxS补体菌株，通过口服途径被接种到怀孕的豚鼠毒力模型和鸡定殖模型中。在这两种情况下，IA3902 luxS突变体都证明完全无法定植肠道。在怀孕模型中，该突变体也未能诱导流产，而野生型菌株是高度流产的。 luxS基因的遗传互补完全恢复了两个模型中的毒力表型。有趣的是，当通过腹膜内途径将有机体接种到豚鼠中时，在luxS突变体和野生型菌株之间未观察到毒力的差异（流产诱导），这表明口服接种后的毒力缺陷可能与细菌感染有关。生物体在肠道外定植和/或移位的缺陷。这些研究提供了第一个直接证据，即使用自然疾病的体内模型，LuxS在空肠弯曲杆菌的毒力中起重要作用。

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