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首页> 外文期刊>Infection and immunity >Loss of Meningococcal PilU Delays Microcolony Formation and Attenuates Virulence In Vivo
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Loss of Meningococcal PilU Delays Microcolony Formation and Attenuates Virulence In Vivo

机译：脑膜炎球菌PilU的丢失延迟了小菌落的形成并减弱了体内的毒力。

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摘要

Neisseria meningitidis is a major cause of sepsis and bacterial meningitis worldwide. This bacterium expresses type IV pili (Tfp), which mediate important virulence traits such as the formation of bacterial aggregates, host cell adhesion, twitching motility, and DNA uptake. The meningococcal PilT protein is a hexameric ATPase that mediates pilus retraction. The PilU protein is produced from the pilT-pilU operon and shares a high degree of homology with PilT. The function of PilT in Tfp biology has been studied extensively, whereas the role of PilU remains poorly understood. Here we show that pilU mutants have delayed microcolony formation on host epithelial cells compared to the wild type, indicating that bacterium-bacterium interactions are affected. In normal human serum, the pilU mutant survived at a higher rate than that for wild-type bacteria. However, in a murine model of disease, mice infected with the pilT mutant demonstrated significantly reduced bacterial blood counts and survived at a higher rate than that for mice infected with the wild type. Infection of mice with the pilU mutant resulted in a trend of lower bacteremia, and still a significant increase in survival, than that of the wild type. In conclusion, these data suggest that PilU promotes timely microcolony formation and that both PilU and PilT are required for full bacterial virulence.
机译：脑膜炎奈瑟菌是全世界败血症和细菌性脑膜炎的主要原因。该细菌表达IV型菌毛（Tfp），其介导重要的毒力特性，例如细菌聚集体的形成，宿主细胞的粘附，抽搐运动和DNA吸收。脑膜炎球菌PilT蛋白是介导菌毛回缩的六聚体ATPase。 PilU蛋白由pilT-pilU操纵子产生，并与PilT具有高度同源性。 PilT在Tfp生物学中的功能已被广泛研究，而PilU的作用仍知之甚少。在这里我们显示，与野生型相比，pilU突变体在宿主上皮细胞上具有延迟的微菌落形成，表明细菌-细菌相互作用受到影响。在正常人血清中，pilU突变体的存活率高于野生型细菌。但是，在疾病的鼠模型中，感染pilT突变体的小鼠表现出明显减少的细菌血细胞计数，并且比感染野生型小鼠的存活率更高。与野生型相比，用pilU突变体感染小鼠导致较低的菌血症趋势，并且存活率仍显着提高。总之，这些数据表明，PilU可促进及时的小菌落形成，并且PilU和PilT都需要充分的细菌毒力。

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