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首页> 外文期刊>Infection and immunity >Identification of Genes Involved in the Expression of Atypical Lipooligosaccharide Structures from a Second Class of Haemophilus ducreyi
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Identification of Genes Involved in the Expression of Atypical Lipooligosaccharide Structures from a Second Class of Haemophilus ducreyi

机译：鉴定涉及第二类杜克嗜血杆菌的非典型脂寡糖结构表达的基因

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摘要

Haemophilus ducreyi is a gram-negative bacterium that is the causative agent of chancroid. Strain 35000HP has been well characterized and is representative of the majority of H. ducreyi strains. Strain 35000HP produces a lipooligosaccharide (LOS) that contains d-glycero-d-manno-heptose in the main oligosaccharide chain extension; the lbgB gene has been shown to encode the dd-heptosyltransferase. The lbgB gene is found in a gene cluster together with the lbgA gene, which encodes for the galactosyltransferase I. These two genes are flanked by two housekeeping genes, rpmE and xthA, encoding the ribosomal protein L31 and the exonuclease III, respectively. Recently, a second group of H. ducreyi strains have been identified. Strain 33921, a representative of the class II strains, produces an LOS that lacks dd-heptose in the oligosaccharide portion of its LOS. To better understand the biosynthesis of the dd-heptose-deficient 33921 LOS, we cloned and sequenced the corresponding lbgAB genomic region from strain 33921. Similar to strain 35000HP, the 33921 genome contains xthA and rpmE. However, between these two genes we identified genes encoding two putative glycosyltransferases that were not highly homologous to the 35000HP lbgAB genes. In this study, we demonstrate that the product of one of these genes encodes a galactosyltransferase. In addition, dot blot hybridization determined that 3 of 35 strains tested had the atypical transferases present, as did 4 strains characterized as class II strains by other criterion. These data indicate that the lbgAB genes can serve as one indicator of the classification of H. ducreyi strains.
机译： Duemyi嗜血杆菌是革兰氏阴性细菌，是类c虫的病原体。菌株35000HP已被很好地表征，代表了大多数 H。 ducreyi 菌株。 35000HP菌株产生的低聚寡糖（LOS）在主要寡糖链延伸中包含d- 甘油 -d- 甘露糖-庚糖;已经证明 lbgB 基因编码dd-庚糖基转移酶。 lbgB 基因与 lbgA 基因一起编码在一个基因簇中，该基因编码半乳糖基转移酶I。这两个基因的两侧是两个管家基因 rpmE 和 xthA ，分别编码核糖体蛋白L31和核酸外切酶III。最近，第二群 H。已经鉴定出杜克雷伊菌株。菌株32921是II类菌株的代表，产生的LOS在其LOS的寡糖部分中缺少dd-庚糖。为了更好地了解缺乏dd-庚糖的33921 LOS的生物合成，我们从菌株33921克隆了相应的 lbgAB 基因组区域并进行了测序。与菌株35000HP相似，33921基因组包含 xthA 和 rpmE 。但是，在这两个基因之间，我们确定了编码两个推定的糖基转移酶的基因，这些酶与35000HP lbgAB 基因不是高度同源。在这项研究中，我们证明了这些基因之一的产物编码半乳糖基转移酶。另外，斑点印迹杂交确定了测试的35个菌株中的3个存在非典型转移酶，按照其他标准，表征为II类菌株的4个菌株也是如此。这些数据表明 lbgAB 基因可以作为 H分类的一个指标。 ducreyi 菌株。

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