Proteomic and Immunoblot Analyses of Bartonella quintana Total Membrane Proteins Identify Antigens Recognized by Sera from Infected Patients

Jenni K. Boonjakuakul; Helen L. Gerns; Yu-Ting Chen; Linda D. Hicks; Michael F. Minnick; Scott E. Dixon; Steven C. Hall; Jane E. Koehler

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Proteomic and Immunoblot Analyses of Bartonella quintana Total Membrane Proteins Identify Antigens Recognized by Sera from Infected Patients

机译：金枪鱼巴尔通体总膜蛋白的蛋白质组学和免疫印迹分析可鉴定感染患者血清识别的抗原

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Bartonella quintana is a fastidious, gram-negative, rod-shaped bacterium that causes prolonged bacteremia in immunocompetent humans and severe infections in immunocompromised individuals. We sought to define the outer membrane subproteome of B. quintana in order to obtain insight into the biology and pathogenesis of this emerging pathogen and to identify the predominant B. quintana antigens targeted by the human immune system during infection. We isolated the total membrane proteins of B. quintana and identified 60 proteins by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and peptide mass fingerprinting. Using the newly constructed proteome map, we then utilized two-dimensional immunoblotting with sera from 21 B. quintana-infected patients to identify 24 consistently recognized, immunoreactive B. quintana antigens that have potential relevance for pathogenesis and diagnosis. Among the outer membrane proteins, the variably expressed outer membrane protein adhesins (VompA and VompB), peptidyl-prolyl cis-trans-isomerase (PpI), and hemin-binding protein E (HbpE) were recognized most frequently by sera from patients, which is consistent with surface expression of these virulence factors during human infection.

机译： Bartonella quintana 是一种挑剔的革兰氏阴性杆状细菌，可引起具有免疫能力的人长时间的菌血症，并导致免疫功能低下的人受到严重感染。我们试图定义 B的外膜亚蛋白质组。 Quintana ，以便深入了解这种新兴病原体的生物学和发病机理，并确定主要的 B。在感染过程中被人类免疫系统靶向的金枪鱼抗原。我们分离了 B的总膜蛋白。二维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和肽质量指纹图谱鉴定了60种蛋白质。使用新构建的蛋白质组图谱，然后我们对21 B血清进行了二维免疫印迹分析。被金枪鱼感染的患者，以识别出24个一致认可的免疫反应性 B。金枪鱼抗原与发病机理和诊断具有潜在的相关性。在外膜蛋白中， v 通常表达为 o 子宫 m 膜 p 蛋白粘附素（VompA和VompB），肽基脯氨酰顺式-反转录-异构酶（PpI）和 h emin- b inding p rotein <患者血清最常识别到em> E （HbpE），这与人类感染期间这些毒力因子的表面表达相一致。 展开▼

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《Infection and immunity》 |2007年第5期|共14页

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Jenni K. Boonjakuakul; Helen L. Gerns; Yu-Ting Chen; Linda D. Hicks; Michael F. Minnick; Scott E. Dixon; Steven C. Hall; Jane E. Koehler;
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中图分类医学免疫学;

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2. Two-dimensional immunoblot analysis of antigenic proteins of Spirometra plerocercoid recognized by human patient sera [J] . Rahman Moizur, Lee Eung-Goo, Bae Young-An . Parasitology International . 2011,第2期

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3. Immunoblot analysis of membrane antigens of Schistosoma mansoni, Schistosoma intercalatum, and Schistosoma haematobium against Schistosoma-infected patient sera [J] . Italo M. Cesari, Diana E. Ballen, L. Mendoza, Parasitology Research . 2010,第5期

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5. Proteomic and Immunoblot Analyses of Bartonella quintana Total Membrane Proteins Identify Antigens Recognized by Sera from Infected Patients [O] . Jenni K. Boonjakuakul, Helen L. Gerns, Yu-Ting Chen, 2007

机译：金枪鱼巴尔通体总膜蛋白的蛋白质组学和免疫印迹分析可鉴定感染患者血清识别的抗原

6. Proteomic and Immunoblot Analyses of Bartonella quintana Total Membrane Proteins Identify Antigens Recognized by Sera from Infected Patients▿ [O] . Boonjakuakul, Jenni K., Gerns, Helen L., Chen, Yu-Ting, 2007

机译：金枪鱼巴尔通体总膜蛋白的蛋白质组学和免疫印迹分析可鉴定感染患者血清识别的抗原▿

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Proteomic and Immunoblot Analyses of Bartonella quintana Total Membrane Proteins Identify Antigens Recognized by Sera from Infected Patients

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