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首页> 外文期刊>Infection and immunity >Outer Surface Protein A Protects Lyme Disease Spirochetes from Acquired Host Immunity in the Tick Vector
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Outer Surface Protein A Protects Lyme Disease Spirochetes from Acquired Host Immunity in the Tick Vector

机译：外表面蛋白A保护壁虱病螺旋体免受虱子载体中获得的宿主免疫

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The Lyme disease spirochete Borrelia burgdorferi alters the expression of outer surface protein (osp) genes as the bacterium cycles between ticks and mammals. OspA is produced as borreliae enter the tick vector and remains a major surface antigen during midgut colonization. To elucidate the role of OspA in the vector, we created an insertional deletion of ospA in strain B31-A3. The ospA mutant infects mice when it is injected intradermally and is acquired by larval ticks fed on these mice, where it persists through the molt to the nymph stage. Bacterial survival rates in artificially infected tick larvae fed on na?ve mice were compared with those in the vector fed on immune mice. The ospA mutant proliferates in larvae if it is exposed to blood from na?ve mice, but it declines in density after larval feeding if the blood is from immune mice. When uninfected larvae are fed on B-cell-deficient mice infected with the ospA mutant, larvae show borrelial densities and persistence that are significantly greater than those fed on infected, immunocompetent mice. We conclude that OspA serves a critical antibody-shielding role during vector blood meal uptake from immune hosts and is not required for persistence in the tick vector.
机译：随着tick虫和哺乳动物之间的细菌循环，莱姆病螺旋体 Borrelia burgdorferi 改变了外表面蛋白（ osp ）基因的表达。 OspA是在疏螺旋体进入壁虱载体时产生的，并且在中肠定居过程中仍是主要的表面抗原。为了阐明OspA在载体中的作用，我们在菌株B31-A3中创建了 ospA 的插入缺失。皮内注射 ospA 突变体会感染小鼠，并通过喂食这些小鼠的幼虫tick来获得，并在整个蜕皮中一直持续到若虫期。将幼稚小鼠喂养的人工感染infected幼虫的细菌存活率与免疫小鼠喂养的载体中的细菌存活率进行了比较。如果暴露于幼稚小鼠的血液中， ospA 突变体会在幼虫中增殖，但是如果血液来自免疫小鼠，则在幼虫摄食后其密度会降低。当将未感染的幼虫喂食被 ospA 突变体感染的B细胞缺陷型小鼠时，幼虫显示出的硼密度和持久性明显高于被感染的具有免疫能力的老鼠。我们得出的结论是，OspA在从免疫宿主摄取载体血粉的过程中起着至关重要的抗体屏蔽作用，并且不需要在壁虱载体中持续存在。

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