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首页> 外文期刊>Infection and immunity >Generation of High-Titer Neutralizing Antibodies against Botulinum Toxins A, B, and E by DNA Electrotransfer
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Generation of High-Titer Neutralizing Antibodies against Botulinum Toxins A, B, and E by DNA Electrotransfer

机译：通过DNA电转移产生针对肉毒杆菌毒素A，B和E的高滴度中和抗体

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Botulinum neurotoxins are known to be among the most toxic known substances. They produce severe paralysis by preventing the release of acetylcholine at the neuromuscular junction. Thus, new strategies for efficient production of safe and effective anti-botulinum neurotoxin antisera have been a high priority. Here we describe the use of DNA electrotransfer into the skeletal muscle to enhance antiserum titers against botulinum toxin serotypes A, B, and E in mice. We treated animals with codon-optimized plasmid DNA encoding the nontoxic but highly immunogenic C-terminal heavy chain fragment of the toxin. By employing both codon optimization and the electrotransfer procedure, the immune response and corresponding neutralizing antiserum titers were markedly increased. The cellular localization of the antigen and the immunization regimens were also shown to increase neutralizing titers to >100 IU/ml. This study demonstrates that DNA electrotransfer is an effective procedure for raising neutralizing antiserum titers to remarkably high levels.
机译：肉毒杆菌神经毒素是已知毒性最高的物质之一。它们通过阻止神经肌肉接头处乙酰胆碱的释放而产生严重的瘫痪。因此，高效生产安全有效的抗肉毒杆菌神经毒素抗血清的新策略已成为当务之急。在这里，我们描述了使用DNA电转移到骨骼肌中来增强小鼠抗肉毒杆菌毒素血清型A，B和E的抗血清效价。我们用密码子优化的质粒DNA处理动物，该质粒DNA编码该毒素的无毒但具有高度免疫原性的C端重链片段。通过同时使用密码子优化和电转移程序，免疫反应和相应的中和抗血清效价显着提高。还显示了抗原的细胞定位和免疫方案可将中和滴度提高至> 100 IU / ml。这项研究表明，DNA电转移是一种将中和抗血清滴度提高到非常高水平的有效方法。

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