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首页> 外文期刊>Infection and immunity >Salmonella enterica Serovar Typhimurium Pathogenicity Island 1-Encoded Type III Secretion System Translocases Mediate Intimate Attachment to Nonphagocytic Cells
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Salmonella enterica Serovar Typhimurium Pathogenicity Island 1-Encoded Type III Secretion System Translocases Mediate Intimate Attachment to Nonphagocytic Cells

机译：肠杆菌沙门氏菌鼠伤寒沙门氏菌致病性岛1-编码的III型分泌系统转座菌介导紧密附着于非吞噬细胞。

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摘要

Delivery of bacterial proteins into mammalian cells by type III secretion systems (TTSS) is thought to require the intimate association of bacteria with target cells. The molecular bases of this intimate association appear to be different in different bacteria involving TTSS components, as well as surface determinants not associated with TTSS. We show here that the protein translocases SipB, SipC, and SipD of the Salmonella enterica serovar Typhimurium pathogenicity island 1 (SPI-1)-encoded TTSS are required for the intimate association of these bacteria with mammalian cells. S. Typhimurium mutant strains lacking any of the translocases were defective for intimate attachment. Immunofluorescence microscopy showed that SipD is present on the bacterial surface prior to bacterial contact with host cells. In contrast, SipB and SipC were detected on the bacterial surface only subsequent to bacterial contact with the target cell. We conclude that the coordinated deployment and interaction between the protein translocases mediate the SPI-1 TTSS-dependent intimate association of S. Typhimurium with host cells.
机译：通过III型分泌系统（TTSS）将细菌蛋白递送到哺乳动物细胞中被认为需要细菌与靶细胞的紧密结合。这种紧密联系的分子基础在涉及TTSS成分的不同细菌以及与TTSS不相关的表面决定簇方面似乎是不同的。我们在这里显示，沙门氏菌血清鼠伤寒沙门氏菌致病岛1（SPI-1）编码的TTSS的蛋白转位酶SipB，SipC和SipD是这些细菌与哺乳动物细胞紧密结合所必需的。 S 。缺乏任何转座酶的鼠伤寒突变株在亲密附着方面存在缺陷。免疫荧光显微镜显示，在细菌与宿主细胞接触之前，SipD存在于细菌表面。相反，仅在细菌与靶细胞接触后才在细菌表面检测到SipB和SipC。我们得出结论，蛋白移位酶之间的协调部署和相互作用介导了 S 的SPI-1 TTSS依赖性紧密联系。鼠伤寒与宿主细胞。

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