...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Sand Fly Saliva Enhances Leishmania amazonensis Infection by Modulating Interleukin-10 Production
【24h】

Sand Fly Saliva Enhances Leishmania amazonensis Infection by Modulating Interleukin-10 Production

机译：沙蝇唾液通过调节白介素10的产生增强亚马逊利什曼原虫的感染

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

After transmission through the bite of female sand flies, Leishmania spp. can cause a broad spectrum of disease manifestations collectively known as leishmaniases. L. amazonensis is endemic in South America, where it causes cutaneous, diffuse cutaneous, and visceral leishmaniasis. In this study, we have provided evidence that salivary gland extracts (SGE) of Lutzomyia longipalpis enhances L. amazonensis infection. BALB/c mice infected intradermally in the ear with 105 metacyclic promastigotes of L. amazonensis together with SGE (equivalent to 0.5 gland) showed an early onset of disease and larger lesions that contained ～3-log-units more parasites than did controls. To determine the potential mechanism underlying this enhancement, we assessed cytokine production via reverse transcriptase PCR and enzyme-linked immunosorbent assay. Mice coinjected with parasites and SGE displayed higher levels of interleukin-10 (IL-10) mRNA in the ear tissues, as well as higher levels of IL-10 in supernatants of restimulated draining lymph node (LN) cells, than did controls. Flow cytometric analysis revealed high frequencies of IL-10-producing CD4+ and CD8+ T cells in the draining LN of mice coinjected with the parasite and SGE. In addition, we examined bone marrow derived-macrophage cultures and detected increased IL-10 but decreased nitric oxide (NO) production in cells exposed to SGE prior to infection with L. amazonensis. Together, these results imply that the sand fly saliva facilitates Leishmania evasion of the host immune system by modulating IL-10 production.
机译：通过雌性沙蝇叮咬传播后，利什曼原虫 spp。可以引起广泛的疾病表现，统称为利什曼病。 L。亚马逊（Amazonensis）在南美洲很流行，会引起皮肤，弥漫性皮肤和内脏利什曼病。在这项研究中，我们提供了证据，证明 Lutzomyia longipalpis 的唾液腺提取物（SGE）增强了 L。亚马逊感染。 BALB / c小鼠的耳内皮内感染了10个 L的meta 5 前环前鞭毛。亚马逊与SGE（相当于0.5腺体）一起显示出疾病的早期发作和较大的病灶，其寄生虫比对照组的寄生虫多约3个对数单位。为了确定这种增强的潜在机制，我们通过逆转录酶PCR和酶联免疫吸附测定评估了细胞因子的产生。与寄生虫和SGE共同注射的小鼠与对照组相比，在耳组织中显示出更高的白细胞介素10（IL-10）mRNA水平，以及在再刺激排水淋巴结（LN）细胞上清液中的IL-10水平更高。流式细胞仪分析显示，与寄生虫和SGE共同注射的小鼠引流LN中产生IL-10的CD4 + 和CD8 + T细胞的频率很高。此外，我们检查了骨髓衍生的巨噬细胞培养物，并发现在感染 L之前，暴露于SGE的细胞中IL-10含量升高，但一氧化氮（NO）产量降低。亚马逊。总之，这些结果表明，沙蝇唾液通过调节IL-10的产生而促进宿主免疫系统的“利什曼原虫”逃避。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号