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Rapid Loss of Motility of Helicobacter pylori in the Gastric Lumen In Vivo

机译：体内胃腔中幽门螺杆菌的动力迅速丧失。

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The human pathogen Helicobacter pylori has infected more than half of the world's population. Nevertheless, the first step of infection, the acute colonization of the gastric mucus, is poorly understood. For successful colonization, H. pylori must retain active motility in the gastric lumen until it reaches the safety of the mucus layer. To identify the factors determining the acute colonization, we inserted bacteria into the stomach of anesthetized Mongolian gerbils. We adjusted the gastric juice to defined pH values of between 2.0 and 6.0 by using an autotitrator. Despite the fact that Helicobacter spp. are known to survive low pH values for a certain time in vitro, the length of time that H. pylori persisted under the assay conditions within the gastric juice in vivo was remarkably shorter. In the anesthetized animal we found H. pylori to be irreversibly immotile in less than 1 min at lumen pH values of 2 and 3. At pH 4 motility was lost after 2 min. However, the period of motility increased to more than 15 min at pH 6. Blocking pepsins in the gastric lumen in vivo by using pepstatin significantly increased the period of motility. It was possible to simulate the rapid in vivo immotilization in vitro by adding pepsins. We conclude that pepsin limits the persistence of H. pylori in the gastric chymus to only a few minutes by rapidly inhibiting active motility. It is therefore likely that this short period of resistance in the gastric lumen is one of the most critical phases of Helicobacter infection.
机译：人类病原体幽门螺杆菌感染了世界一半以上的人口。然而，人们对感染的第一步，即胃粘液的急性定植知之甚少。为了成功定植， H。幽门螺杆菌必须在胃腔内保持活跃的运动，直到达到粘液层的安全性。为了确定决定急性定植的因素，我们将细菌插入了麻醉的蒙古沙鼠的胃中。我们使用自动滴定仪将胃液的pH值调整为2.0至6.0。尽管 Helicobacter spp。已知低pH值可以在体外一定时间（Hem的持续时间）中存活。幽门螺杆菌在胃液中持续存在的条件下明显缩短。在麻醉的动物中，我们发现了 H。幽门螺杆菌在管腔pH值为2和3的不到1分钟内不可逆地运动。在pH 4下2分钟后丧失了运动性。但是，在pH值为6时，运动时间增加到15分钟以上。通过使用胃抑素在体内阻止胃蛋白酶抑制胃蛋白酶在体内的运动时间显着增加了。通过添加胃蛋白酶可以模拟体外快速体内动员。我们得出结论，胃蛋白酶限制了 H的持久性。通过快速抑制活跃的运动能力，使胃食道中的幽门螺杆菌仅持续几分钟。因此，胃管腔的这种短暂抵抗很可能是幽门螺杆菌感染的最关键阶段之一。

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