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Role of the Distal sarA Promoters in SarA Expression in Staphylococcus aureus

机译:远端sarA启动子在金黄色葡萄球菌SarA表达中的作用

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The global regulatory locus sarA comprises a 375-bp open reading frame that is driven by three promoters, the proximal P1 and distal P3 and P2 promoters. We mutated the weaker P3 and P2 promoters to ascertain the effect of the change on SarA protein and target gene expression. Our results indicated that the solely active P1 promoter led to a lower SarA protein level, which has an effect on agr transcription and subsequently had corresponding effects on hla, sspA, and spa transcription, probably in both agr-independent and agr-dependent manners.
机译:全球调控位点 sarA 包含一个375 bp的开放阅读框,由三个启动子(近端P1和远端P3和P2启动子)驱动。我们突变了较弱的P3和P2启动子,以确定变化对SarA蛋白和靶基因表达的影响。我们的结果表明,单独激活的P1启动子导致较低的SarA蛋白水平,这对 agr 转录有影响,随后对 hla sspA也有相应的影响 spa 转录,可能以 agr 独立和 agr 独立的方式出现。

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