Acid-Induced Activation of the Urease Promoters Is Mediated Directly by the ArsRS Two-Component System of Helicobacter pylori

Michael Pflock; Simone Kennard; Isabel Delany; Vincenzo Scarlato; Dagmar Beier

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Acid-Induced Activation of the Urease Promoters Is Mediated Directly by the ArsRS Two-Component System of Helicobacter pylori

机译：幽门螺杆菌的ArsRS两组分系统直接介导尿素酶启动子的酸诱导激活。

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The nickel-containing enzyme urease is an essential colonization factor of the human gastric pathogen Helicobacter pylori which enables the bacteria to survive the low-pH conditions of the stomach. Transcription of the urease genes is positively controlled in response to increasing concentrations of nickel ions and acidic pH. Here we demonstrate that acid-induced transcription of the urease genes is mediated directly by the ArsRS two-component system. Footprint analyses identify binding sites of the phosphorylated ArsR response regulator within the ureA and ureI promoters. Furthermore, deletion of a distal upstream ArsR binding site of the ureA promoter demonstrates its role in acid-dependent activation of the promoter. In addition, acid-induced transcription of the ureA gene is unaltered in a nikR mutant, providing evidence that pH-responsive regulation and nickel-responsive regulation of the ureA promoter are mediated by independent mechanisms involving the ArsR response regulator and the NikR protein.

机译：含镍酶脲酶是人胃病原体幽门螺杆菌的必不可少的定居因子，可使细菌在胃的低pH条件下存活。响应于镍离子浓度的增加和酸性pH的增加，尿素酶基因的转录受到了积极的控制。在这里，我们证明了酸诱导的脲酶基因转录是直接由ArsRS两组分系统介导的。足迹分析确定了 ureA 和 ureI 启动子中磷酸化ArsR反应调节剂的结合位点。此外，删除 ureA 启动子的远端上游ArsR结合位点表明了其在启动子的酸依赖性激活中的作用。此外，在 nikR 突变体中，酸诱导的 ureA 基因的转录没有改变，这提供了 ureA的pH响应调节和镍响应调节的证据。启动子由涉及ArsR反应调节剂和NikR蛋白的独立机制介导。 展开▼

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《Infection and immunity》 |2005年第10期|共9页

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Michael Pflock; Simone Kennard; Isabel Delany; Vincenzo Scarlato; Dagmar Beier;
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中图分类医学免疫学;

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1. Acid-Induced Activation of the Urease Promoters Is Mediated Directly by the ArsRS Two-Component System of Helicobacter pylori [J] . Michael Pflock, Simone Kennard, Isabel Delany, Infection and immunity . 2005,第10期

机译：幽门螺杆菌的ArsRS两组分系统直接介导尿素酶启动子的酸诱导激活。

2. The HP0165-HP0166 Two-Component System (ArsRS) Regulates Acid-Induced Expression of HP1186 α-Carbonic Anhydrase in Helicobacter pylori by Activating the pH-Dependent Promoter [J] . Yi Wen, Jing Feng, David R. Scott, Journal of bacteriology . 2007,第6期

机译：HP0165-HP0166两组分系统（ArsRS）通过激活pH依赖型启动子来调节酸性诱导的HP1186α-碳酸酐酶在幽门螺杆菌中的表达

3. Determinants of the regulation of Helicobacter pylori adhesins include repeat sequences in both promoter and coding regions as well as the two-component system ArsRS [J] . Catherine R. Acio-Pizzarello, Abigail A. Acio, Edward J. Choi, Journal of Medical Microbiology: An Official Journal of the Pathological Society of Great Britain and Ireland . 2017,第6期

机译：幽门螺杆菌粘附素调控的决定因素包括启动子和编码区以及两组分系统ArsRS中的重复序列

4. Epitope mapping and antigenic evaluation of Helicobacter pylori Urease subunit beta fragment [C] . Ehsan Raoufi, Hassan Akrami, Behzad Khansarinejad, International Workshop on Mining and Analyzing Social Networks for Decision Support;International Workshop on Social Network Analysis in Applications;International Workshop on Dynamics in Networks;Social Media and Risk;International Symposium on Network Enabled Health Informatics, Biomedicine and Bioinformatics;International Symposium on Foundations of Open Source Intelligence and Security Informatics;International Symposium on Foundations and Applications of Big Data Analytics;Multiplex Attributed Networks Mining;IEEE/ACM International Conference on Advances in Social Networks Analysis and Mining . 2015

机译：幽门螺杆菌脲酶亚基β片段的表位作图和抗原性评估

5. Helicobacter pylori colonization of the mouse gastric mucosa: The Entner-Doudoroff pathway and development of a promoter-trapping system. [D] . Wanken, Amy E. 2003

机译：幽门螺杆菌在小鼠胃黏膜的定植：Entner-Doudoroff途径和启动子捕获系统的发展。

6. Acid-Induced Activation of the Urease Promoters Is Mediated Directly by the ArsRS Two-Component System of Helicobacter pylori [O] . Michael Pflock, Simone Kennard, Isabel Delany, 2005

机译：幽门螺杆菌的ArsRS两组分系统直接介导尿酸启动子的酸诱导激活。

7. Acid-Induced Activation of the Urease Promoters Is Mediated Directly by the ArsRS Two-Component System of Helicobacter pylori [O] . Pflock, Michael, Kennard, Simone, Delany, Isabel, 2005

机译：幽门螺杆菌的ArsRS两组分系统直接介导尿酸启动子的酸诱导激活。

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3. 兴奋性氨基酸受体的激活介导冷水应激诱导的tau蛋白磷酸化 [J] . 武凤英 ,冯琼 ,程敏 . 生物化学与生物物理进展 . 2010,第005期

4. 幽门螺杆菌尿素酶减毒鼠伤寒杆菌疫苗诱导小鼠黏膜免疫应答的研究 [J] . 刘晓峰 ,胡家露 ,权启镇 . 中国现代医学杂志 . 2004,第021期

5. 与启动子序列相同的单链寡聚脱氧核苷酸拮抗胰岛素对人α1(Ⅰ)型胶原启动子的激活 [J] . 徐振兴 ,伍严安 ,龙瑞斌 . 福建医科大学学报 . 2005,第003期

6. 幽门螺杆菌尿素酶A、过氧化氢酶的诱导表达 [C] . 廖文俊 . 2000年度全国中西医结合消化系统疾病学术交流会 . 2000

7. 双组分系统ArsRS参与幽门螺杆菌多耐药机制研究 [A] . 李文 . 2021

1. 能同时响应水杨酸SA和茉莉酸JA诱导的诱导启动子及其应用 [P] . 中国专利： CN103589731B . 2015.11.04

2. 能同时响应水杨酸SA和茉莉酸JA诱导的诱导启动子及其应用 [P] . 中国专利： CN103589731A . 2014-02-19

3. Method for detection the Helicobacter pylori bacteria and the urease test for detection of the Helicobacter pylori bacteria [P] . 外国专利： PL215889B1 . 2014-02-28

机译：幽门螺杆菌的检测方法和幽门螺杆菌的尿素酶检测

4. Method for detection the Helicobacter pylori bacteria and the urease test for detection of the Helicobacter pylori bacteria [P] . 外国专利： PL392212A1 . 2012-02-27

机译：幽门螺杆菌的检测方法和幽门螺杆菌的尿素酶检测

5. Antibody enzyme against urease of Helicobacter pylori, a gene encoding the same, the gene transformants that have been introduced, and therapeutic agents and infection prevention agent of Helicobacter pylori-infected patients who use them [P] . 外国专利： JP4330947B2 . 2009-09-16

机译：编码幽门螺杆菌的基因的抗幽门螺杆菌脲酶的抗体酶，已导入的基因转化体以及感染幽门螺杆菌的患者的治疗剂和感染预防剂

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Acid-Induced Activation of the Urease Promoters Is Mediated Directly by the ArsRS Two-Component System of Helicobacter pylori

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