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首页> 外文期刊>Infection and immunity >cDNA Microarray Analysis of Host-Pathogen Interactions in a Porcine In Vitro Model for Toxoplasma gondii Infection
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cDNA Microarray Analysis of Host-Pathogen Interactions in a Porcine In Vitro Model for Toxoplasma gondii Infection

机译：弓形虫感染猪体外模型中宿主-病原体相互作用的cDNA芯片分析

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Toxoplasma gondii induces the expression of proinflammatory cytokines, reorganizes organelles, scavenges nutrients, and inhibits apoptosis in infected host cells. We used a cDNA microarray of 420 annotated porcine expressed sequence tags to analyze the molecular basis of these changes at eight time points over a 72-hour period in porcine kidney epithelial (PK13) cells infected with T. gondii. A total of 401 genes with Cy3 and Cy5 spot intensities of ≥500 were selected for analysis, of which 263 (65.6%) were induced ≥2-fold (expression ratio, ≥2.0; P ≤ 0.05 [t test]) over at least one time point and 48 (12%) were significantly down-regulated. At least 12 functional categories of genes were modulated (up- or down-regulated) by T. gondii. The majority of induced genes were clustered as transcription, signal transduction, host immune response, nutrient metabolism, and apoptosis related. The expression of selected genes altered by T. gondii was validated by quantitative real-time reverse transcription-PCR. These results suggest that significant changes in gene expression occur in response to T. gondii infection in PK13 cells, facilitating further analysis of host-pathogen interactions in toxoplasmosis in a secondary host.
机译：弓形虫弓形虫诱导感染的宿主细胞中促炎细胞因子的表达，重组细胞器，清除营养并抑制细胞凋亡。我们使用420个带注释的猪表达序列标签的cDNA微阵列分析了感染 T的猪肾上皮（PK13）细胞在72小时内八个时间点上这些变化的分子基础。刚地。总共选择了401个Cy3和Cy5点强度≥500的基因进行分析，其中263个（65.6％）被诱导≥2倍（表达率，≥2.0; P ≤0.05 [ t 测试]）在至少一个时间点上显着下调了48个（12％）。至少12种功能类别的基因被 T调控（上调或下调）。刚地。大部分诱导基因聚类为转录，信号转导，宿主免疫反应，营养代谢和凋亡相关。所选基因的表达被 T改变。实时定量逆转录-PCR验证了弓形虫。这些结果表明，响应 T，基因表达发生了显着变化。刚体感染PK13细胞，有助于进一步分析次级宿主弓形体中的宿主-病原体相互作用。

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