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首页> 外文期刊>Infection and immunity >Mice Lacking Components of Adaptive Immunity Show Increased Brucella abortus virB Mutant Colonization
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Mice Lacking Components of Adaptive Immunity Show Increased Brucella abortus virB Mutant Colonization

机译：缺乏适应性免疫的小鼠显示增加了流产布鲁氏菌virB突变定植

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The Brucella abortus type IV secretion system (T4SS), encoded by the virB genes, is essential for survival in mononuclear phagocytes in vitro. In the mouse model, a B. abortus virB mutant was initially able to colonize the spleen at the level of the wild type for approximately 3 to 5 days, which coincided with the development of adaptive immunity. To investigate the relationship between survival in macrophages cultivated in vitro and persistence in tissues in vivo, we tested the ability of mutant mice lacking components of adaptive immunity to eliminate the virB mutant from the spleen during a mixed infection with the B. abortus wild type. Ifng?/? or β2m?/? mice were able to clear the virB mutant to the same degree as control mice. However, spleens of Rag1?/? mice and Igh6?/? mice were more highly colonized by the virB mutant than control mice after 14 to 21 days, suggesting that, in these mice, there is not an absolute requirement for the T4SS to mediate persistence of B. abortus in the spleen. Macrophages isolated from Igh6?/? mice killed the virB mutant to the same extent as macrophages from control mice, showing that the reduced ability of these mice to clear the virB mutant from the spleen does not correlate with diminished macrophage function in vitro. These results show that in the murine model host, the T4SS is required for persistence beyond 3 to 5 days after infection and suggest that the T4SS may contribute to evasion of adaptive immune mechanisms by B. abortus.
机译：由 virB 基因编码的流产布鲁氏菌型IV分泌系统（T4SS）对于体外单核吞噬细胞的存活至关重要。在鼠标模型中，为 B。流产virB 突变体最初能够在野生型水平上定植脾脏约3-5天，这与适应性免疫的发展相吻合。为了研究体外培养的巨噬细胞的存活与体内组织的持久性之间的关系，我们测试了缺乏适应性免疫成分的突变小鼠在混合感染期间从脾脏清除 virB 突变体的能力。 B。流产野生型。 Ifng ？/？或β 2 m ？/？< / sup>小鼠能够清除 virB 突变体的程度与对照小鼠相同。但是， Rag1 ？/？小鼠和 Igh6 ？/？小鼠的脾脏在<在14到21天后，em> virB 突变体比对照小鼠突变，这表明，在这些小鼠中，对T4SS介导 B的持久性没有绝对要求。脾中流产。从 Igh6 ？/？小鼠中分离出的巨噬细胞杀死 virB 突变体的程度与对照小鼠的巨噬细胞相同，表明这些小鼠的能力降低从脾脏清除 virB 突变体的小鼠与体外巨噬细胞功能减弱无关。这些结果表明，在鼠模型宿主中，T4SS对于感染后3至5天的持久性是必需的，并且表明T4SS可能有助于逃避 B的适应性免疫机制。流产。

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