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首页> 外文期刊>Infection and immunity >Evaluation of the Ability of Streptococcus agalactiae Strains Isolated from Genital and Neonatal Specimens To Bind to Human Fibrinogen and Correlation with Characteristics of the fbsA and fbsB Genes
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Evaluation of the Ability of Streptococcus agalactiae Strains Isolated from Genital and Neonatal Specimens To Bind to Human Fibrinogen and Correlation with Characteristics of the fbsA and fbsB Genes

机译：生殖和新生儿标本分离无乳链球菌菌株与人血纤蛋白原结合的能力及其与fbsA和fbsB基因特征的相关性

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The ability of 111 Streptococcus agalactiae strains to bind to human fibrinogen was quantified. We correlated the percentages of bacteria that bound to immobilized fibrinogen with fibrinogen-binding (fbs) gene characteristics of strains and with clinical origin, serotypes, and phylogenetic positions of strains. Percentages varied from 0.4 to 29.9%. Fifty-five strains (49.5%) had the fbsB gene sensu stricto described by Gutekunst et al. (Infect. Immun., 72:3495-3504, 2004), allowing adhesion to human fibrinogen, and all of the other strains had an fgag variant gene. Ninety strains (81.1%) had a fbsA gene and 55 of them also had the fbsB gene. The other 21 strains (18.9%) had a truncated form of fbsA without the fbsB gene sensu stricto. The numbers of 48-nucleotide repeat sequences (rs) in the fbsA gene varied from 2 to 26. The population of strains with the highest ability to bind to human fibrinogen significantly more frequently had the fbsB gene sensu stricto and 4 to 7 rs in the fbsA gene (P < 0.05). However, the single strain that carried the highest number of rs (26 rs) in the fbsA gene showed high fibrinogen-binding activity (24.3%). Strains exhibiting significantly higher levels of binding to human fibrinogen belonged to a phylogenetic group of strains associated with neonatal meningitis, currently known as the ST-17 clone, that is mostly composed of serotype III strains. These findings indicate that S. agalactiae strains possess a wide variety of fbs gene content that markedly influences the ability of strains to bind to human fibrinogen. Variations in the configuration and the expression of the Fbs proteins may therefore partly explain the variability of virulence in S. agalactiae species.
机译：定量了111株无乳链球菌菌株与人纤维蛋白原结合的能力。我们将与固定化纤维蛋白原结合的细菌的百分比与菌株的纤维蛋白原结合（ fbs ）基因特征以及菌株的临床起源，血清型和系统发生位置相关联。百分比从0.4到29.9％不等。 55株（49.5％）具有Gutekunst等人描述的 fbsB 基因sensu stricto。 （Infect。Immun。，72：3495-3504，2004），其允许与人纤维蛋白原的粘附，并且所有其他菌株均具有 fgag 变体基因。 90个菌株（81.1％）具有 fbsA 基因，其中55个也具有 fbsB 基因。其他21个菌株（占18.9％）的截短形式为 fbsA ，而没有 fbsB 基因。 fbsA 基因中48个核苷酸的重复序列（rs）从2到26不等。与人血纤蛋白原结合能力最高的菌株群中显着更频繁地具有 fbsB 基因sensustricto和 fbsA 基因中的4至7 rs（ P <0.05）。然而，在 fbsA 基因中携带最高rs（26 rs）数量的单个菌株显示出高的纤维蛋白原结合活性（24.3％）。表现出明显更高水平的与人纤维蛋白原结合的菌株属于与新生儿脑膜炎相关的系统发育菌株，目前被称为ST-17克隆，主要由血清型III菌株组成。这些发现表明 S。无乳杆菌菌株具有多种 fbs 基因含量，显着影响菌株与人血纤蛋白原的结合能力。因此，Fbs蛋白的构型和表达的变化可能部分解释了 S中毒力的变异性。无乳杆菌种。

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