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Chlamydial Effector Proteins Localized to the Host Cell Cytoplasmic Compartment

机译：衣原体效应蛋白定位于宿主细胞的细胞质隔室

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Disease-causing microbes utilize various strategies to modify their environment in order to create a favorable location for growth and survival. Gram-negative bacterial pathogens often use specialized secretion systems to translocate effector proteins directly into the cytosol of the eukaryotic cells they infect. These bacterial proteins are responsible for modulating eukaryotic cell functions. Identification of the bacterial effectors has been a critical step toward understanding the molecular basis for the pathogenesis of the bacteria that use them. Chlamydiae are obligate intracellular bacterial pathogens that have a type III secretion system believed to translocate virulence effector proteins into the cytosol of their host cells. Selective permeabilization of the eukaryotic cell membrane was used in conjunction with metabolic labeling of bacterial proteins to identify chlamydial proteins that localize within the cytosol of infected cells. More than 20 Chlamydia trachomatis and C. pneumoniae proteins were detected within the cytoplasmic compartment of infected cells. While a number of cytosolic proteins were shared, others were unique to each species, suggesting that variation among cytosolic chlamydial proteins contributes to the differences in the pathogenesis of the chlamydial species. The spectrum of chlamydial proteins exported differed concomitant with the progress of the developmental cycle. These data confirm that a dynamic relationship exists between Chlamydia and its host and that translocation of bacterial proteins into the cytosol is developmentally dependent.
机译：致病微生物利用各种策略来改变其环境，以便为生长和生存创造有利的位置。革兰氏阴性细菌病原体通常使用专门的分泌系统将效应子蛋白直接转移到它们感染的真核细胞的胞质溶胶中。这些细菌蛋白负责调节真核细胞功能。鉴定细菌效应子是迈向了解使用它们的细菌发病机理的分子基础的关键步骤。衣原体是专性的细胞内细菌病原体，其具有III型分泌系统，据信该系统可将毒力效应蛋白转运到其宿主细胞的细胞质中。真核细胞膜的选择性通透化与细菌蛋白的代谢标记结合使用，以鉴定位于感染细胞胞质内的衣原体蛋白。超过20个沙眼衣原体和 C。在感染细胞的细胞质隔室中检测到肺炎蛋白。尽管共享了许多胞质蛋白，但其他蛋白对于每个物种而言都是唯一的，这表明胞质衣原体蛋白之间的差异有助于衣原体物种在发病机理上的差异。衣原体蛋白的输出谱随发育周期的进展而不同。这些数据证实了衣原体与其宿主之间存在动态关系，并且细菌蛋白向胞质溶胶中的转运是发育依赖性的。

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