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首页> 外文期刊>Infection and immunity >Surface Proteome of “Mycobacterium avium subsp. hominissuis” during the Early Stages of Macrophage Infection
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Surface Proteome of “Mycobacterium avium subsp. hominissuis” during the Early Stages of Macrophage Infection

机译：“鸟分枝杆菌亚种”的表面蛋白质组。在巨噬细胞感染的早期阶段

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“Mycobacterium avium subsp. hominissuis” is a robust and pervasive environmental bacterium that can cause opportunistic infections in humans. The bacterium overcomes the host immune response and is capable of surviving and replicating within host macrophages. Little is known about the bacterial mechanisms that facilitate these processes, but it can be expected that surface-exposed proteins play an important role. In this study, the selective biotinylation of surface-exposed proteins, streptavidin affinity purification, and shotgun mass spectrometry were used to characterize the surface-exposed proteome of M. avium subsp. hominissuis. This analysis detected more than 100 proteins exposed at the bacterial surface of M. avium subsp. hominissuis. Comparisons of surface-exposed proteins between conditions simulating early infection identified several groups of proteins whose presence on the bacterial surface was either constitutive or appeared to be unique to specific culture conditions. This proteomic profile facilitates an improved understanding of M. avium subsp. hominissuis and how it establishes infection. Additionally, surface-exposed proteins are excellent targets for the host adaptive immune system, and their identification can inform the development of novel treatments, diagnostic tools, and vaccines for mycobacterial disease.
机译：“鸟分枝杆菌亚种。 “ hominissuis”是一种强大而普遍的环境细菌，可引起人类机会性感染。该细菌克服了宿主的免疫反应，能够在宿主巨噬细胞内存活和复制。关于促进这些过程的细菌机制知之甚少，但是可以预料，表面暴露的蛋白质将发挥重要作用。在这项研究中，表面暴露的蛋白质的选择性生物素化，链霉亲和素亲和纯化和shot弹枪质谱用于表征鸟分枝杆菌亚种的表面暴露的蛋白质组。人参。该分析检测了在鸟分枝杆菌亚种细菌表面暴露的100多种蛋白质。人参。在模拟早期感染的条件之间对表面暴露的蛋白质进行比较，发现了几组蛋白质，它们在细菌表面上的存在是组成性的或对于特定培养条件而言似乎是独特的。该蛋白质组学概况有助于更好地了解鸟分枝杆菌亚种。人参及其如何建立感染。此外，表面暴露的蛋白质是宿主适应性免疫系统的极佳靶标，其鉴定可为分枝杆菌疾病的新型治疗方法，诊断工具和疫苗的开发提供信息。

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